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US 2003/0224009 A1Published application
Bacterial expression systems
AssigneeThe University of Queensland
InventorsTamsin Deborah Terry, Michael Paul Jennings
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Abstract
An inducible expression system is provided that includes an inducible ansB promoter co-dependently regulatable by cyclic AMP and anaerobiosis. The expression system is particularly suited to chromosomal expression of immunogenic proteins in attenuated bacterial vaccines. Protein expression from an E. coli-derived ansB promoter is particularly effective in a Salmonella host bacterium.
Claims29
An expression vector comprising a promoter co-dependently regulatable by cyclic AMP and anaerobiosis, which expression vector is capable of at least one of:
- (i) being chromosomally-integrated and maintained in a bacterial cell;
- and
- (ii) being maintained extrachromosomally in a bacterial cell.
Dependencies are inferred from the claim text.