Loading patent…
OwnerFlorine Johanna van Milligen, Johannes Bernardus, Wilhelmus, Joseph Cornelissen +1inventors
InventorsFlorine Johanna van Milligen, Johannes Bernardus, Wilhelmus, Joseph Cornelissen, Bernard Adri Bokhout
Estimating the term…
The invention relates to an ex vivo animal or challenge model as a method to identify protective (recombinant) proteins and rapidly measure protective immunity in intestinal segments directed against parasites and vaccines directed against parasitic infections. The invention further relates to vaccines directed against infection with parasites, such as Fasciola hepatica, which vaccines contain protective (recombinant) proteins identified and shown to be protective in studies using the ex vivo model. The invention further relates to protective (recombinant) proteins obtained from newly excysted juveniles (NEJ) of Fasciola hepatica. The protective (recombinant) protein corresponding to an NEJ protein has an apparent molecular weight of 32 kD and an N-terminal amino acid sequence comprising the sequence XXDVSWPFWDRMYNY (SEQ ID NO: 1).
A protective protein, or antigenic fragment thereof, said protein comprising an amino acid sequence derived from a proregion of a parasite protease.
A method for measuring protective immunity directed against a parasite, said method comprising using an ex vivo animal or challenge model to measure said protective immunity directed against a parasite.
A method for testing a vaccine, the method comprising using an ex vivo animal or challenge model to measure a protective immunity directed against a parasite.
A diagnostic test measuring an antibody directed against an immunodominant, parasite-specific epitope on a cathepsin-like protease.
A vaccine composition comprising:
A vaccine composition comprising:
A vaccine composition comprising:
An isolated protein comprising a polypeptide from a pro-region of a cathepsin L protease obtained from excretory/secretory products of newly excysted juvenile stages of Fasciola hepatica.
Dependencies are inferred from the claim text.