US 2003/0221965 A1Published application

Capillary electrophoresis device

AssigneesHitachi High-Technologies Corporation, Applera Corporation

InventorsTaisaku Seino, Tomoyuki Sakai, Miho Ozawa, Masaya Kojima, Eric S. Nordman

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Abstract

An electrophoresis device is disclosed that is capable of reducing waves occurring in a fluid in which the capillary tips of a capillary assay are immersed. A plurality of partition plate members can be provided in a container containing the fluid to divide the surface of the fluid into several sections. The container can be transported at a high speed, and the capillary tips can be immersed in the fluid quickly, thereby preventing resolution degradation caused by a long period of exposure of the capillary tips to air. Throughput of sample analysis can also be improved because it is not necessary to wait until waves disappear before conducting an analysis.

Claims22

Dependent claims

An electrophoresis device comprising:

a plurality of capillaries each being filled with a separation medium for sample separation, each capillary having an injecting end portion for introducing a sample and a light illumination portion capable of being illuminated with an excitation light;
a power source device capable of applying a voltage to a current-carrying path extending at least between the injecting end portion and the light illumination portion of each of the plurality of capillaries;
a fluorescence excitation device capable of illuminating the light illumination portion of each of the plurality of capillaries with light;
a light detection device capable of detecting light emitted from the light illumination portion of each of the plurality of capillaries;
a fluid container capable of holding a fluid into which the injecting end portions of each of the plurality of capillaries is immersed, the fluid container being provided with a fluid resistance member;
and
a container transportation device capable of transporting the fluid container.

4 more elements

An electrophoresis device comprising:

a plurality of capillaries each being filled with a separation medium for sample separation, each capillary having an injecting end portion for introducing a sample and a light illumination portion capable of being illuminated with an excitation light;
a power source device capable of applying a voltage to a current-carrying path extending at least between the injecting end portion and the light illumination portion of each of the plurality of capillaries;
a fluorescence excitation device capable of illuminating the light illumination portion of each of the plurality of capillaries with light;
a light detection device capable of detecting light emitted from the light illumination portion of each of the plurality of capillaries;
a sample container capable of holding a plurality of samples, the sample container having a sample container usage position in which the samples held in the sample container are brought into contact with the injecting end portion of each of the plurality of capillaries;
a fluid container capable of holding a fluid into which the injecting end portion of each of the plurality of capillaries are immersed;
a sample container storage device capable of storing the sample container;
and
a transportation device capable of transporting the sample container from the sample container storage device to the sample container usage position within 14 seconds after the injecting end portion of each of the plurality of capillaries is exposed to air.

6 more elements

An electrophoresis device comprising:

a plurality of capillaries each capable of being filled with a separation medium for sample separation, each capillary including an injecting end portion for introducing a sample and a light illumination portion capable of being illuminated with an excitation light;
a power source device capable of applying a voltage to a current-carrying path extending at least between the injecting end portion and the light illumination portion of each of the plurality of capillaries;
a fluorescence excitation device capable of illuminating the light illumination portion of each of the plurality of capillaries with light;
a light detection device capable of detecting light emitted from the light illumination portion of each of the plurality of capillaries;
a sample container capable of holding a plurality of samples, the sample container including a sample container usage position in which the samples held in the sample container are brought into contact with the injecting end portion of each of the plurality of capillaries;
a fluid container capable of holding a fluid into which the injecting end portion of each of the plurality of capillaries are immersed;
a sample container storage device capable of storing the sample container;
and
a transportation device capable of transporting the sample container from the sample container storage device to the sample container usage position within 14 seconds after the injecting end portion of each of the plurality of capillaries is exposed to air.

6 more elements

Dependencies are inferred from the claim text.