Obtusifoliol 14a-demethylase
OwnerLeslie T. Harvell, Brian McGonigle +1inventors
InventorsLeslie T. Harvell, Brian McGonigle, Stephen O. Pember
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a cytochrome P450, more specifically obtusifoliol 14α-demethylase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of obtusifoliol 14α-demethylase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of obtusifoliol 14α-demethylase in a transformed host cell.
Claims36
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having obtusifoliol 14α-demethylase activity, wherein the polypeptide has an amino acid sequence of at least 80% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:6, or
- (b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
An isolated polypeptide having obtusifoliol 14α-demethylase activity, wherein the polypeptide has an amino acid sequence of at least 80% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:6.
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having obtusifoliol 14α-demethylase activity, wherein the polypeptide has an amino acid sequence of at least 95% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:4, or
- (b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
An isolated polypeptide having obtusifoliol 14α-demethylase activity, wherein the polypeptide has an amino acid sequence of at least 95% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:4.
Dependencies are inferred from the claim text.