Feline immunoglobulin E molecules and related methods
AssigneeHeska Corporation
InventorsCatherine McCall, Eric Weber
Life of this patent
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Abstract
The invention relates to: nucleic acid molecules encoding the light chain and heavy chain of feline immunoglobulin E (IgE), including species-specific regions of feline IgE; proteins encoded by the nucleic acid molecules; inhibitors to the nucleic acids and proteins; antibodies to the proteins; cells transformed with the nucleic acid molecules; assays employing the transformed cells, nucleic acids, antibodies and/or proteins or portions thereof; methods for treating IgE-mediated responses (ie. allergy) using the materials provided; methods for eliciting an immune response to IgE and kits containing the nucleic acid molecules, proteins or derivatives thereof (ie. antibodies).
Claims21
An isolated nucleic acid molecule encoding a portion of a feline IgE heavy chain protein, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence which has more than 82% identity to a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO 1;
- and SEQ ID NO 28, wherein said identity can be determined using the DNAsis computer program and default parameters;
- (b) a nucleic acid sequence which encodes a feline heavy chain protein which has more than 76% identity to an amino acid sequence selected from the group consisting of:
- SEQ ID NO 2;
- and SEQ ID NO 29, wherein said identity can be determined using the DNAsis computer program and default parameters;
- (c) a nucleic acid sequence which encodes and a feline heavy chain protein encoded by an allelic variant of a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO 1;
- and SEQ ID NO 28;
- and
- (d) a nucleic acid sequence which has more than 90% identity to a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO 3;
- SEQ ID NO 4;
- SEQ ID NO 6;
- SEQ ID NO 7;
- SEQ ID NO 9;
- SEQ ID NO 10;
- SEQ ID NO 12;
- SEQ ID NO 13;
- SEQ ID NO 15;
- SEQ ID NO 16;
- SEQ ID NO 18;
- and SEQ ID NO 31;
- and
- (e) a nucleic acid molecule fully complementary to a nucleic acid molecule selected from the group consisting of:
- a nucleic acid molecule of (a);
- a nucleic acid molecule of (b);
- and a nucleic acid molecule of (c).
An isolated protein encoding a portion of a feline IgE heavy chain molecule, wherein said protein comprises an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence encoded by a nucleic acid sequence which has more than 82% identity to a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO 1;
- and SEQ ID NO:
- 28, wherein said identity can be determined using the DNAsis computer program and default parameters;
- (b) an amino acid sequence which has more than 76% identity to an amino acid sequence selected from the group consisting of:
- SEQ ID NO 2;
- and SEQ ID NO:
- 29, wherein said identity can be determined using the DNAsis computer program and default parameters;
- (c) an amino acid sequence encoded by an allelic variant of a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO 1;
- and SEQ ID NO:
- 28;
- and
- (d) an amino acid sequence encoded by a a nucleic acid sequence which has more than 90% identity to a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO 3;
- SEQ ID NO 4;
- SEQ ID NO 6;
- SEQ ID NO 7;
- SEQ ID NO 9;
- SEQ ID NO 10;
- SEQ ID NO 12;
- SEQ ID NO 13;
- SEQ ID NO 15;
- SEQ ID NO 16;
- SEQ ID NO 18;
- and SEQ ID NO 31.
An isolated nucleic acid molecule encoding a portion of a feline IgE light chain protein, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence which has more than 84% identity to SEQ ID NO 19, and wherein said identity can be determined using the DNAsis computer program and default parameters;
- (b) a nucleic acid sequence which encodes a feline heavy chain protein selected from the group consisting of:
- a feline heavy chain protein which has more than 61% identity to SEQ ID NO 20, wherein said identity can be determined using the DNAsis computer program and default parameters;
- and a feline heavy chain protein encoded by an allelic variant of SEQ ID NO 19;
- (c) a nucleic acid sequence which has more than 95% identity to a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO 23;
- and SEQ ID NO 25;
- and
- (d) a nucleic acid molecule fully complementary to a nucleic acid molecule selected from the group consisting of:
- a nucleic acid molecule of (a);
- a nucleic acid molecule of (b);
- and a nucleic acid molecule of (c).
An isolated protein encoding a portion of a feline IgE light chain molecule, wherein said protein comprises an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence encoded by a nucleic acid sequence which has more than 84% identity to SEQ ID NO 19, and wherein said identity can be determined using the DNAsis computer program and default parameters;
- (b) an amino acid sequence which has more than 61% identity to SEQ ID NO 20, wherein said identity can be determined using the DNAsis computer program and default parameters;
- (c) an amino acid sequence encoded by an allelic variant of SEQ ID NO 19;
- and
- (d) a nucleic acid sequence which has more than 95% identity to a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO 23;
- and SEQ ID NO 25.
Dependencies are inferred from the claim text.