Intravascular delivery of non-viral nucleic acid
OwnerSean D. Monahan, Jon A. Wolff +4inventors
InventorsSean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
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Abstract
Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
Claims12
1) A process for delivering a polynucleotide complexed with a compound into a parenchymal cell of a mammal, comprising:
- a) making the polynucleotide-compound complex wherein the compound is selected from the group consisting of amphipathic compounds, polymers and non-viral vectors;
- b) inserting the polynucleotide into a mammalian vessel;
- c) increasing the permeability of the vessel;
- and,
- d) delivering the polynucleotide to the parenchymal cell.
9) A process for transfecting genetic material into a mammalian cell, comprising:
- a) designing the genetic material for transfection;
- b) inserting the genetic material into a mammalian blood vessel;
- c) increasing permeability of the blood vessel;
- d) delivering the genetic material to the mammalian cell;
- and,
- e) altering endogenous properties of the cell.
Dependencies are inferred from the claim text.