Isolated polynucleotides and methods of promoting a morphology in a fungus
AssigneeBattelle Memorial Institute
InventorsLinda L. Lasure, Ziyu Dai
Life of this patent
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Abstract
The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology.
Claims45
An isolated polynucleotide molecule comprising a polynucleotide sequence that is differentially expressed in a native fungus exhibiting a pellet morphology relative to the native fungus exhibiting a filamentous morphology.
A method of promoting a morphology in a fungus comprising:
- providing a recombinant polynucleotide molecule;
- transforming a fungus with the recombinant polynucleotide;
- and
- expressing a polypeptide encoded by the polynucleotide sequence to promote a fungal morphology.
An isolated polynucleotide molecule comprising a nucleotide sequence set forth in any one of SEQ ID NOs.:1, 4, 6, 8, 12, 16, 18, 22, 24, 26, 28, 33, 36 and 37.
A recombinant polynucleotide construct comprising a polynucleotide sequence consisting of at least 30 consecutive nucleotides sequence of any one of SEQ ID NOs.:1, 4, 6, 8, 12, 16, 18, 22, 24, 26, 28, 33, 36, 37, and sequences complimentary to SEQ ID NOs.:1, 4, 6, 8, 12, 16, 18, 22, 24, 26, 28, 33, 36, and 37.
A method of promoting a morphology in a fungus comprising:
- providing a recombinant polynucleotide comprising at least 30 consecutive nucleotides of any one of SEQ ID NOs.:1, 4, 6, 8, 12, 16, 18, 22, 24, 26, 28, 33, 36, and 37;
- transforming a fungus with the recombinant polynucleotide;
- and
- expressing a polypeptide encoded by the polynucleotide sequence to promote a fungal morphology.
A method of promoting a morphology in a fungus comprising:
- providing a recombinant polynucleotide comprising an antisense orientated sequence that is complimentary to at least part of a gene;
- transforming a fungus with the recombinant polynucleotide;
- and
- suppressing expression of the gene utilizing transcription products produced by expression of the recombinant polynucleotide, the suppression promoting the morphology.
An isolated polynucleotide molecule encoding a polypeptide comprising an amino acid sequence set forth in any one of SEQ ID NOs.:2, 5, 7, 9, 13, 17, 19, 23, 25, 27, 29 and 34.
A method of promoting a morphology in a fungus comprising:
- providing a recombinant polynucleotide comprising isolated polynucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in any one of SEQ ID NOs.:2, 5, 7, 9, 13, 17, 19, 23, 25, 27, 29 and 34;
- transforming a fungus with the recombinant polynucleotide;
- and
- expressing a polypeptide encoded by the polynucleotide sequence to promote a fungal morphology.
A method of enhancing a bioprocess utilizing a fungus, comprising:
- producing a transformed fungus by transforming a fungus with a recombinant polynucleotide molecule comprising a polynucleotide sequence set forth in any of SEQ ID NOs.:22, 24, 26, 28, 33, 36 and 37, linked operably to a promoter;
- and
- expressing a polypeptide encoded by the polynucleotide sequence, the polypeptide promoting pellet morphology, the pellet morphology enhancing a bioprocess relative to the bioprocess utilizing a filamentous morphology of the transformed fungus.
A method of enhancing a bioprocess utilizing a fungus, comprising:
- producing a transformed fungus by transforming a fungus with a recombinant polynucleotide molecule comprising a polynucleotide sequence complimentary to any one of SEQ ID NOs.:
- 1, 4, 6, 8, 12, 16, and 18, linked operably to a promoter in antisense orientation;
- transcribing the polynucleotide sequence to produce polynucleotide transcripts;
- and
- hybridizing the transcripts to mRNA to suppress gene expression and promote pellet morphology, the pellet morphology enhancing a bioprocess relative to the bioprocess utilizing a filamentous morphology of the transformed fungus.
A method of enhancing a bioprocess utilizing a fungus, comprising:
- producing a transformed fungus by transforming a fungus with a recombinant polynucleotide molecule comprising a polynucleotide sequence complimentary to any one of SEQ ID NOs.:
- 22, 24, 26, 28, 33, 36, and 37, linked operably to a promoter in antisense orientation;
- transcribing the polynucleotide sequence to produce polynucleotide transcripts;
- and
- hybridizing the transcripts to mRNA to suppress gene expression and promote filamentous morphology, the filamentous morphology enhancing a bioprocess relative to the bioprocess utilizing a pellet morphology of the transformed fungus.
An isolated polynucleotide molecule comprising at least a 10 nucleotide segment of the sequence set forth in SEQ ID NO. 1;
- the at least 10 nucleotide segment comprising at least one codon that codes for a different amino acid relative to the corresponding codon set forth in SEQ ID NO. 3.
An isolated polynucleotide molecule that encodes a protein having a first level of expression in a fungus exhibiting a filamentous morphology and having a decreased level or no expression in the fungus exhibiting a pellet morphology.
An isolated polynucleotide molecule that encodes a protein having a first level of expression in a fungus exhibiting a pellet morphology and having a decreased level or no expression in the fungus exhibiting a filamentous morphology.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- an amino acid sequence having greater than 31% identity to SEQ ID NO.10;
- an amino acid sequence having greater than 56% identity to SEQ ID NO.14;
- an amino acid sequence having greater than 66% identity to SEQ ID NO.20;
- an amino acid sequence having greater than 97% identity with SEQ ID NO.2;
- an amino acid sequence containing at least 10 consecutive amino acids as set forth in any one of SEQ ID NOs. 5, 7, 10, 17, 19, 23, 25, 27, and 29;
- an amino acid sequence containing greater than 16 consecutive amino acids as set forth in SEQ ID NO.14;
- an amino acid sequence containing greater than 25 consecutive amino acids as set forth in SEQ ID NO.14;
- an amino acid sequence set forth in any one of SEQ ID NOs. 30, 31, and 32;
- and an amino acid sequence homologous to any one of the sequences set forth in SEQ ID NOs.:2, 5, 7, 9, 13, 17, 19, 23, 27, 29 and 34.
Dependencies are inferred from the claim text.