Dehydroascorbate reductase ("DHAR") genes and their uses
AssigneeThe Regents of the University of California
InventorDaniel R. Gallie
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Abstract
The present invention is generally related to plant genetic engineering. In particular, the invention is directed to new dehydroascorbate reductase (“DHAR”) genes useful in modulating ascorbic acid levels in plants.
Claims48
An isolated nucleic acid encoding a DHAR polypeptide comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 1, wherein the sequence is not SEQ ID NO:
- 9, SEQ ID NO:
- 13 or SEQ ID NO:
- 15.
A recombinant expression cassette comprising a promoter sequence operably linked to a nucleic acid encoding a DHAR polypeptide comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 1.
A transgenic plant comprising a recombinant expression cassette comprising a plant promoter sequence operably linked to a nucleic acid encoding a DHAR polypeptide comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 1.
A method of increasing ascorbic acid levels in a plant comprising introducing a construct comprising a promoter operably linked to a nucleic acid encoding a DHAR polypeptide comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 1.
A method of decreasing ascorbic acid levels in a plant comprising introducing a construct comprising a promoter operably linked to a nucleic acid encoding a DHAR polypeptide comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 1.
An isolated DHAR polypeptide comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 1, wherein the sequence is not SEQ ID NO:
- 9, SEQ ID NO:
- 13 or SEQ ID NO:
- 15.
Dependencies are inferred from the claim text.