Methods for enhancing targeted gene alteration using oligonucleotides
OwnerEric B. Kmiec, Howard B. Gamper +2inventors
InventorsEric B. Kmiec, Howard B. Gamper, Michael C. Rice, Li Liu
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Abstract
Methods are presented for enhancing the efficiency of oligonucleotide-medidated repair or alteration of genetic information. The methods comprise using cells or cell-free extracts having altered levels or activity of at least one protein from the RAD52 epistasis group, the mismatch repair group or the nucleotide excision repair group. Kits and compositions are also presented.
Claims20
A method of enhancing oligonucleotide-mediated gene alteration efficiency comprising using a cell or a cell-free extract with reduced levels or activity of at least one protein selected from the group consisting of a homolog, ortholog or paralog of RAD1, RAD51, RAD52, RAD57 and PMS1.
A kit comprising at least one cell or at least one cell-free extract wherein the cell or cell-free extract comprises reduced protein levels or activity of at least one protein selected from the group consisting of a homolog, ortholog or paralog or RAD1, RAD51, RAD52, RAD57 and PMS1.
A kit comprising a collection of at least two different cells wherein each cell has reduced protein levels or activity of a different protein selected from the group consisting of a homolog, ortholog or paralog or RAD1, RAD51, RAD52, RAD57 and PMS1.
Dependencies are inferred from the claim text.