Biologically active Eph family ligands
OwnerSamuel Davis, Nicholas W. Gale +1inventors
InventorsSamuel Davis, Nicholas W. Gale, George D. Yancopoulos
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Abstract
A novel ligand (Efl-6) that binds the Elk subfamily of Eph receptors is identified, and methods for making the soluble Elf-6 ligand in biologically active form is described. A cDNA clone encoding this novel protein enables production of the recombinant protein, which is useful to support neuronal and other eph receptor-bearing cell populations.
Claims16
An isolated and purified nucleic acid molecule encoding Efl-6 protein wherein the sequence of said nucleic acid is selected from the group consisting of:
- (a) the sequence of the DNA encoding mature Efl-6 protein contained in the plasmid pBluescriptSK14 Efl-6 as deposited with the American Type Culture Collection on Oct. 19, 1995 and designated as 97319;
- (b) the sequence of the DNA encoding mature Efl-6 protein as set forth in FIG. 1;
- (c) DNA sequences that hybridize under moderately stringent conditions to the DNA of (a) or (b) and which encode a protein that binds a receptor belonging to the Elk subfamily of Eph receptors;
- and
- (d) DNA sequences that are degenerate as a result of the genetic code to a DNA sequence of (a), (b), or (c) and which encode an Efl-6 protein that binds a member of the Elk subclass of Eph receptors.
Isolated and purified mature Efl-6 protein having an amino acid sequence as set forth in FIG. 1.
An isolated nucleic acid encoding the extracellular domain of Efl-6 (sEfl-6) having a sequence selected from the following:
- (a) the sequence set forth from about nucleotide 274 to about nucleotide 873 of FIG. 1;
- and
- (b) a sequence which encodes the extracellular domain of Efl-6 as set forth in FIG. 1.
Dependencies are inferred from the claim text.