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US 2003/0215917 A1Published application
Assay for evaluation of activity of compounds against HCV using a novel detection system in the HCV replicon
OwnerMingjun Huang, Yongnian Sun +3inventors
InventorsMingjun Huang, Yongnian Sun, Wengang Yang, Yongsen Zhao, Joanne Fabrycki
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Abstract
The present invention relates to a cell-based HTS assay for evaluation of antiviral activity of compounds against HCV using HCV replicon.
Claims20
A method for analysis of the antiviral and cell toxicity activity upon Hepatitis C virus of test compound(s) comprising the steps of:
- a. constructing a Hepatitis C replicon system containing a gene required for said replicon to replicate (replicon);
- b. transfecting and propagating cells which contain said replicon;
- selecting stably transfected clones;
- c. growing and propagating said stably transfected clones;
- plating said cells and adding test compound(s) to wells containing the cells transfected with the replicon;
- d. incubating the transfected cells in the presence of the test compound(s);
- quantifying a gene product protein produced by the replicon during the incubation period as a measure of antiviral activity;
- and
- e. quantifying a marker for cellular protein expression level produced by the cells during the incubation period as a measure of test compound toxicity.
6 more elements
A method for simultaneous analysis of the antiviral and cell toxicity activity upon Hepatitis C virus of test compound(s) comprising the steps of:
- a. constructing a Hepatitis C replicon system containing a gene required for said replicon to replicate (replicon);
- b. transfecting and propagating cells which contain said replicon;
- c. selecting stably transfected clones;
- d. growing and propagating said stably transfected clones;
- e. plating said cells and adding test compound(s) to wells containing the cells transfected with the replicon;
- f. incubating the transfected cells in the presence of the test compound(s);
- g. simultaneously quantifying the amount of a gene product protein produced by the replicon during the incubation period and the amount of a marker for cellular protein expression level produced by the cells during the incubation period.
4 more elements
Dependencies are inferred from the claim text.