Method for cloning and expression of PpuMI restriction endonuclease and PpuMI methylase in E. coli
AssigneeNew England Biolabs, Inc.
InventorsJames Samuelson, Shuang-Yong Xu
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Abstract
The present invention relates to recombinant DNA encoding the PpuMI restriction endonuclease as well as PpuMI methylase, expression of PpuMI restriction endonuclease and PpuMI methylase in E. coli cells containing the recombinant DNA.
Claims6
Isolated DNA encoding the PpuMI restriction endonuclease, wherein the isolated DNA is obtainable from Pseudomonas putida.
A recombinant DNA vector comprising a vector into which a DNA segment encoding the PpuMI restriction endonuclease has been inserted.
Isolated DNA encoding the PpuMI restriction endonuclease and PpuMI methylase, wherein the isolated DNA is obtainable from ATCC No. ______.
Dependencies are inferred from the claim text.