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US 2003/0215906 A1Published application
Recombinant bacillus proteases and uses thereof
AssigneeEnzymes Technology Limited
InventorBoon Leong Lim
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Abstract
The present invention provides genetically engineered Bacillus strains that can secrete large amount of Bacillus proteases in the extracellular culture medium. More particularly, this invention relates to a process of producing recombinant protease molecules of Bacillus origin in a Bacillus subtilis strain 168, utilizing a strong prophage promoter.
Claims23
A process for production of Bacillus protease that comprises a recombinant Bacillus strain transformed with an expression vector comprising
- a. A promoter region of the ORF19 of the bacteriophage phi-105 (SEQ. ID No 14), and
- b. A gene from Bacillus strains encoding said protease (SEQ. ID Nos. 7 or 9).
A method for producing a polypeptide, comprising:
- (a) cultivating a Bacillus cell in a medium conducive for the production of a polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising a Φ105MU331 promoter in which the Φ105MU331 promoter sequence is operably linked to a nucleic acid sequence encoding the polypeptide;
- and
- (b) isolating the polypeptide from the cultivation medium.
Dependencies are inferred from the claim text.