Altered thermostability of enzymes
OwnerJay M. Shortinventor
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Abstract
Provided is a method of screening gene libraries derived from a mixed population of organisms for a bioactivity or biomolecule of interest. The mixed population of organisms can be a cultured population or an uncultured population from, for example, the environment. Also provided are methods of screening isolates or enriched populations of organisms, which isolates include a population that is spatially, temporally, or hierarchical, for example, of a particular species, genus, family, or class of organisms. Identified clones containing a biomolecule or bioactivity of interest can be further variegated or the DNA contained in the clone can be variegated to create novel biomolecules or bioactivities of interest.
Claims41
A method for obtaining a bioactive protein having a thermostability that is altered as compared to that of the corresponding wild-type protein, comprising:
- a) variegating a nucleic acid sequence encoding the wild-type protein;
- and
- b) comparing the bioactivity after variegation with the bioactivity of the wild-type protein, wherein a difference in the bioactivity is indicative of an effect of sequence variegation, thereby providing the a bioactive protein having a thermostability that is altered as compared to that of the corresponding wild-type protein.
Dependencies are inferred from the claim text.