Screening apparatus and method for making
AssigneeClark-MXR, Inc.
InventorWilliam G. Clark
Life of this patent
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Abstract
An apparatus and method for fabricating a one or two-dimensional microassays with a matrix of sites favorably disposed for screening substances such as biomolecules, chemicals or cells as described. The method includes drilling a matrix of wells or through-holes in a glass or similar material using a laser. The drilling creates a region favorably disposed towards binding a molecule or cell. A microassay plate includes a substrate and at least one hole in the substrate containing an immobilized reactant bound to an interior surface of the hole. An array of holes having chemically different immobilized reactants is provided. Holes may be drilled using one or more pulses of light of extremely short duration to create a surface in a localized area that preferentially binds to material.
Claims114
A substrate for selectively attracting particles comprising:
- a surface;
- a particle attracting receptor adjacent to the surface;
- and
- at least a portion of the receptor having a positive charge.
The substrate of claim 32 comprising a binding means for controlling the binding of a cell membrane to the receptor.
A method for fabricating a microassay plate comprising:
- directing at least one pulse of light having a pulse width less than 100 ps from a first laser to at least one localized area on the surface of the plate to form a receptor.
A method of making a substrate favorably disposed towards holding a cell comprising:
- providing a substrate having a surface;
- directing at least one pulse of light having a pulse width less than 100 ps from a first laser to at least one localized area on the surface of the plate to form a receptor area, void, or hole characterized by an affinity for a cell.
A method of creating an array of microstructures in a material for use in molecular sequencing comprising:
- directing at least one pulse of light having a pulse width less than 100 ps. from a first laser to at least one localized area to form a receptor.
A method of creating a receptor in a substrate for use cellular electrophysiology comprising:
- directing at least one pulse of light having a pulse width less than 100 ps. from a first laser to at least one localized area on the surface of the plate to form a cell receptor.
Dependencies are inferred from the claim text.