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US 2003/0215853 A1Published application
Template-drive processes for synthesizing polymers and components related to such processes
AssigneeEmory University
InventorsDavid Lynn, Xiaoyu Li
Life of this patent
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Abstract
Template-directed processes for synthesizing polymers are presented in which a sequence-specific polymer is generated from a corresponding sequence-specific template. The generated polymer has a chain length that corresponds to the chain length of the template.
Claims23
A template-directed process for synthesizing polymers, the process comprising:
- (a) providing a sequence-specific deoxyribonucleic acid (DNA) template having binding sites, the template further having a specific chain length;
- and
- (b) generating a sequence-specific polymer along the DNA template, the sequence-specific polymer having a chain length that corresponds to the specific chain length of the template, the sequence-specific polymer defining a sequence that complements the specific sequence defined by the DNA template, the generating of the sequence-specific polymer comprising:
- (b1) providing modified nucleosides having the hydroxyl at the 5′-carbon replaced with an amine, the modified nucleosides further having the hydroxyl at the 3′-carbon replaced with an acetaldehyde;
- (b2) pre-arranging the modified nucleosides along the sequence-specific DNA template such that each of the modified nucleosides are bound to their respective complementary binding site on the DNA template;
- (b3) forming an imine between adjacent modified nucleosides by reacting the amine of one of the adjacent modified nucleosides with the acetaldehyde of the other of the adjacent modified nucleosides;
- and
- (b4) reducing the imine to form an amine between the adjacent modified nucleosides.
5 more elements
A template-directed process for synthesizing polymers, the process comprising:
- replacing the hydroxyl at the 5′-carbon of a nucleoside with an amine;
- and
- replacing the hydroxyl at the 3′-carbon of the nucleoside with an acetaldehyde.
A template-directed process for synthesizing polymers, the process comprising:
- providing a template having a specific chain length, the template defining a specific sequence;
- and
- generating a polymer along the template through a condensation reaction, the polymer having a chain length that corresponds to the specific chain length of the template, the polymer defining a sequence that corresponds to the specific sequence defined by the template.
A nucleoside monomer comprising:
- an amine at the 5′-carbon, the amine replacing a hydroxyl;
- and
- an acetaldehyde at the 3′-carbon, the acetaldehyde replacing a hydroxyl.
A nucleoside oligomer comprising:
- a first reactive end comprising a modified nucleoside having an amine chemically bonded to the 5′-carbon;
- and
- a second reactive end comprising a modified nucleoside having an acetaldehyde chemically bonded to the 3′-carbon.
A polymer comprising:
- adjacent monomers;
- and
- a linker interposed between the adjacent monomers, the linker being selected from a group consisting of:
- an amine;
- and
- an amide.
3 more elements
A polymer comprising:
- adjacent monomers;
- and
- a linker interposed between the adjacent monomers, the linker being formed as a result of a condensation reaction.
Dependencies are inferred from the claim text.