Selective extraction of DNA from groups of cells
OwnerTodd William Billeinventor
Life of this patent
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Abstract
The invention is in the area of selective extraction of DNA from groups of cells. Selective lysis of a particular cell type within a cellular mixture is performed and then the mixture is separated with a filter that allows the DNA from the lysed cells to flow through the filter, while not allowing the unlysed cells to pass through, thereby selectively extracting the DNA from a particular cell type. In one specific embodiment, spermatozoa DNA can be isolated from biological samples which also contain epithelial cells. Methods and kits are also provided which allow for the sequential extraction of DNA from mixtures of cells. The DNA in the sample can be from human, animal or vegetal origin, or any combination of human, animal or vegetal DNA.
Claims50
A method of isolating DNA from a heterogenous mixture of cells comprising:
- (a) providing a sample containing a heterogeneous mixture of cells that includes a first cell type;
- (b) selectively lysing the first cell type within the mixture of cells;
- (c) allowing the lysed mixture that includes the DNA from the first cell type to flow through a size exclusion filter;
- (d) collecting the filtrate that contains the DNA from the first cell type.
A method of isolating DNA from a heterogenous mixture of cells comprising:
- (a) providing a sample containing a heterogeneous mixture of cells that includes at least a first and second cell type;
- (b) selectively lysing the first cell type within the mixture of cells;
- (c) allowing the lysed mixture that includes DNA from the first cell type to flow through a size exclusion filter;
- (d) collecting the filtrate that contains the DNA from the first cell type;
- (e) separately collecting the intact heterogeneous mixture of cells that includes at least the second cell type;
- (f) selectively lysing the second cell type within the mixture;
- (g) allowing the lysed mixture that includes DNA from the second cell type to flow through a size exclusion filter;
- and
- (h) collecting the filtrate that contains the DNA from the second cell type.
A kit comprising (i) wells with filters that are larger than DNA and smaller than intact cells;
- and (ii) reagents for the selective lysis of female cells followed by the lysis of sperm cells.
Dependencies are inferred from the claim text.