Method for nucleic acid extraction and nucleic acid purification
OwnerMichael G. Lorenzinventor
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Abstract
The invention relates to the use of multivalent cations and chelating agents for nucleic acid extraction and nucleic acid purification with silica-based supporting materials, especially with clay minerals, sand and clay mineral-sand mixtures. Thus a universal method is provided for purifying nucleic acids from each kind of nucleic-containing material in any quantities.
Claims33
Method for the isolation and/or purification of nucleic acids, characterised in that nucleic-acid-containing material (sample)
- a) for the adsorption of nucleic acids, is brought into contact with a supporting material in the presence of a salt of a multivalent cation,
- b) the supporting material is washed at least once, and
- c) the nucleic acids are isolated, by adding an aqueous solution of a chelating agent suitable for the cation to the supporting material for desorption of the nucleic acids, and separating the aqueous, nucleic-acid-containing solution from the supporting material.
Use of clay minerals for the isolation of nucleic acids from nucleic-acid-containing material and/or for the purification of nucleic acids.
Use of a buffer containing a salt of one or more multivalent cations, for binding nucleic acids to silica-based supporting materials.
Dependencies are inferred from the claim text.