Multicomponent meningococcal vaccine
AssigneeMicrobiological Research Authority
InventorsAndrew Robinson, Andrew Richard Gorringe, Michael John Hudson, Karen Margaret Reddin
Life of this patent
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Abstract
A composition is provided comprising N. meningitidis outer membrane vesicles, wherein said outer membrane vesicles are enriched with at least one antigenic component. The composition is suitable for use in vaccines and for treatment of infection, particularly meningococcal infection, demonstrating a broad spectrum of protection. A number of preferred antigenic components are described and include antigenic proteins and proteoglycans derived from N. meningitidis.
Claims31
A composition comprising N. meningitidis outer membrane vesicles, wherein said outer membrane vesicles are enriched with at least one antigenic component.
A vaccine composition comprising outer membrane vesicles from a first strain of N. meningitidis, as well as an antigenic component from a second strain of N. meningitidis different from the first, and a pharmaceutically acceptable carrier.
A method of manufacture of a composition, comprising:
- (a) extracting an antigenic component from an outer membrane of a bacteria, and preparing an aqueous solution of said antigenic component;
- (b) extracting outer membrane vesicles from a culture of N. meningitidis, and preparing an aqueous solution of said outer membrane vesicles;
- (c) obtaining a pharmaceutically acceptable carrier;
- and
- (d) admixing the solution prepared in (a), the solution prepared in (b) and the carrier obtained in (c).
A method of manufacture of a composition, comprising:
- (a) recombinantly expressing a DNA that encodes an antigenic component in a bacteria;
- (b) extracting said antigenic component from the outer membrane of said bacteria, and preparing an aqueous solution of said antigenic component;
- (c) extracting outer membrane vesicles from a culture of N. meningitidis, and preparing an aqueous solution of said outer membrane vesicles;
- (d) obtaining a pharmaceutically acceptable carrier;
- and admixing the solution prepared in (b), the solution prepared in (c) and the carrier obtained in (d).
A method of manufacture of a composition, comprising:
- (a) recombinantly expressing a DNA that encodes an antigenic component in N. meningitidis;
- (b) extracting outer membrane vesicles from said N. meningitidis, and preparing an aqueous solution of said outer membrane vesicles, wherein said outer membrane vesicles comprise said antigenic component;
- (c) obtaining a pharmaceutically acceptable carrier;
- and
- a. (d) admixing the solution prepared in (b), with the carrier obtained in (c).
A method of preventing N. meningitidis infection in an animal, comprising administering an effective dose of a composition comprising an outer membrane vesicle and an antigenic component selected from the group consisting of Cu,Zn-superoxide dismutase;
- neisserial surface protein A (NspA);
- porA;
- OMP85;
- FrpB;
- PilQ;
- Hsf;
- HemK;
- sodC;
- mafA;
- N-acetyl glutamate synthetase;
- and macrophage infectivity potentiator-related protein, and a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.