Method of modulating angiogenesis
OwnerKyung Hwan Seul, Keum Yee Kang +1inventors
InventorsKyung Hwan Seul, Keum Yee Kang, Eric C. Beyer
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Abstract
Disclosed are methods for regulating angiogenesis using gap junctions. Regulator molecules are vascular connexins. The methods comprise administering to the animals one or more connexin recombinant viruses containing connexin genes. Modulating angiogenesis includes the inhibition or induction of angiogenesis.
Claims18
A method of modulating angiogenesis comprising administering a gap junction polypeptide to endothelial cells.
A method of modulating angiogenesis comprising
- a) generating a recombinant viral or plasmid vector comprising a DNA sequence encoding a member of a connexin family of polypeptides operatively linked to a promoter;
- b) transfecting in vitro a population of cultured cells with said recombinant vector, resulting in a population of transfected cells;
- and
- c) transplanting said transfected cells to a mammalian host, such that expression of said DNA sequence within the mammal results in modulating angiogenesis.
A method of inhibiting proliferation of endothelial cells comprising administering to the endothelial cells connexin37 polypeptide or a variant thereof.
A method of inhibiting growth or proliferation of endothelial cells, comprising:
- a) generating a recombinant viral or plasmid vector comprising a DNA sequence encoding a member of a connexin family of polypeptides operatively linked to a promoter;
- b) transfecting in vitro a population of cultured cells with said recombinant vector, resulting in a population of transfected cells;
- and
- c) transplanting said transfected cells to a mammalian host, such that expression of said DNA sequence within the mammal results in inhibition of endothelial cell growth or proliferation.
A method of promoting growth or proliferation of endothelial cells, comprising administering to the endothelial cells a connexin polypeptide or a variant thereof.
A method of promoting growth or proliferation of endothelial cells, comprising:
- a) generating a recombinant viral or plasmid vector comprising a DNA sequence encoding a member of a connexin family of polypeptides operatively linked to a promoter;
- b) transfecting in vitro a population of cultured cells with said recombinant vector, resulting in a population of transfected cells;
- and
- c) transplanting said transfected cells to a mammalian host, such that expression of said DNA sequence within the mammal results in promotion of endothelial cell growth or proliferation.
A method of treating angiogenesis related disease comprising administering to a mammal in need thereof a therapeutically effective amount of a gap junction polypeptide.
Dependencies are inferred from the claim text.