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US 2003/0213693 A1Published application
Microscale electrophoresis devices for biomolecule separation and detection
AssigneeThe Regents of the University of Michigan
InventorsPonnambalam Selvaganapathy, Mark A. Burns, David T. Burke, Carlos H. Mastrangelo
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Abstract
The present invention relates to microfabrication and utilization of microscale electrophoresis devices as well as the separation and detection of biomolecules in microscale electrophoresis devices. The device of the present invention utilizes novel fabrication and detection methods.
Claims20
A device, comprising:
- i) a substrate;
- ii) one or more electrophoresis microchannels on said substrate;
- iii) a sieving matrix disposed within said one or more channels;
- iv) one or more reservoirs in fluidic communication with said one or more channels;
- and
- v) a detector positioned inline with respect to at least one of said one or more channels, said detector comprised of first, second and third electrodes.
3 more elements
A method of fabricating of a capillary electrophoretic device, comprising:
- i) providing:
- a) a support platform and, b) etching means;
- ii) applying a passivation layer to said support platform;
- iii) applying a base layer to said passivation layer;
- iv) applying first, second and third electrodes in a configuration for detection to said base layer;
- v) applying a photoresist layer to said base layer and said electrodes;
- vi) applying a top layer to said photoresist layer, said base layer and said electrodes;
- and,
- vi) removing said photoresist layer with said etching means to produce a capillary electrophoresis channel with an inline detector, said detector comprising said first, second and third electrodes.
6 more elements
Dependencies are inferred from the claim text.