Homogentisate prenyl transferase ("HPT") nucleic acids and polypeptides, and uses thereof
AssigneeMonsanto Technology LLC
InventorsHenry E. Valentin, Tyamagondlu V. Venkatesh, Karunanandaa Balasulojini
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Abstract
The present invention is in the field of plant genetics and biochemistry. More specifically, the present invention relates to genes and polypeptides associated with the tocopherol biosynthesis pathway, namely those encoding homogentisate prenyl transferase activity, and uses thereof.
Claims53
A substantially purified nucleic acid molecule encoding an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 5, 9-11, 57-58 and 90.
A substantially purified polypeptide molecule comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 5, 9-11, 57-58 and 90.
An antibody capable of specifically binding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 5, 9-11, 57-58, and 90.
A transformed plant comprising an introduced nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 5, 9-11, 43-44, 57-58 and 90.
A transformed plant comprising an introduced first nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 5, 9-11, 43-44, 57-58, and 90, and complements thereof, and an introduced second nucleic acid molecule encoding an enzyme selected from the group consisting of tyrA, prephenate dehydrogenase, tocopherol cyclase, dxs, dxr, GGPPS, HPPD, AANT1, ID1, GGH, GMT, tMT2, MT1, GCPE, and complements thereof.
A transformed plant comprising a nucleic acid molecule comprising an introduced promoter region which functions in plant cells to cause the production of an mRNA molecule, wherein said introduced promoter region is linked to a transcribed nucleic acid molecule having a transcribed strand and a non-transcribed strand, wherein said transcribed strand is complementary to a nucleic acid molecule encoding a polypeptide selected from the group consisting of SEQ ID NOs:
- 5, 9-11, 43-44, 57-58, and 90, and wherein said transcribed nucleic acid molecule is linked to a 3′ non-translated sequence that functions in the plant cells to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA sequence.
A method of producing a plant having a seed with an increased total tocopherol level comprising:
- (A) transforming said plant with an introduced nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 5, 9-11, 43-44, 57-58 and 90, and (B) growing said transformed plant.
A seed derived from a transformed plant comprising an introduced nucleic acid molecule encoding a polypeptide comprising an amino acid selected from the group consisting of SEQ ID NOs:
- 5, 9-11, 43-44, 57-58, and 90.
A seed derived from a transformed plant comprising an introduced first nucleic acid molecule encoding an introduced polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 5, 9-11, 43-44, 57-58, and 90 and an introduced second nucleic acid encoding an enzyme selected from the group consisting of tyrA, prephenate dehydrogenase, tocopherol cyclase, dxs, dxr, GGPPS, HPPD, AANT1, ID1, GGH, tMT2, MT1, GCPE, GMT, and complements thereof.
A substantially purified polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 39-42, 46-49, and 92-95, wherein said amino acid sequence is not derived from a nucleic acid molecule that is derived from Nostoc punctiforme, Anabaena, Synechocystis, Zea mays, Glycine max, Arabidopsis thaliana, Oryza sativa, Trichodesmium erythraeum, Chloroflexus aurantiacus, wheat, leek, canola, cotton, or tomato.
A substantially purified nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 39-42, 46-49, and 92-95 wherein said nucleic acid molecule is not derived from Nostoc punctiforme, Anabaena, Synechocystis, Zea mays, Glycine max, Arabidopsis thaliana, Oryza sativa, Trichodesmium erythraeum, Chloroflexus aurantiacus, wheat, leek, canola, cotton, or tomato.
A plant transformed with a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 39-42, 46-49, and 92-95, wherein said nucleic acid molecule is not derived from Nostoc punctiforme, Anabaena, Synechocystis, Zea mays, Glycine max, Arabidopsis thaliana, Oryza sativa, Trichodesmium erythraeum, Chloroflexus aurantiacus, wheat, leek, canola, cotton, or tomato.
A substantially purified nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 31, 34-36, 59-60, and 91.
A substantially purified polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 39-42, 46-49, and 92-95, wherein said amino acid sequence does not comprise any of the amino acid sequences set forth in sequence listings in WO00/68393, WO 00/63391, WO 01/62781, or WO 02/33060, and does not comprise SEQ ID NOs 1-11, 43-45, 57-58, 61-62, and 90 from the present application.
A substantially purified nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 39-42, 46-49, and 92-95, wherein said nucleic acid molecule does not comprise any of nucleic acid sequences set forth in sequence listings in WO00/68393, WO 00/63391, WO 01/62781, or WO 02/33060, does not comprise the nucleic acid sequence with Genebank Accession Nos. AI 897027 or AW 563431, and does not comprise SEQ ID NOs:
- 27-36, 59-60, 88-89, and 91 of the present application.
The nucleic acid molecule of claim 44, wherein the polypeptide further comprises more than one amino acid sequence selected from the group consisting of SEQ ID NOs:
- 39-42, 46-49, and 92-95.
A plant transformed with a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 39-42, 46-49, and 92-95, wherein said nucleic acid molecule does not comprise any of nucleic acid sequences set forth in sequence listings in WO 00/68393, WO 00/63391, WO 01162781, or WO 02/33060, does not comprise does not comprise the nucleic acids sequence with Genebank Accession Nos. Al 897027 or AW 563431 and does not comprise SEQ ID NOs:
- 27-36, 59-60, 88-89, and 91 of the present application.
A substantially purified polypeptide with homogentisate prenyl transferase activity comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 43-44.
A substantially purified nucleic acid molecule encoding a polypeptide with homogentisate prenyl transferase activity comprising an amino acid sequence selected from the group consisting of SEQ ID NOs. 43-44.
Homogentisate prenyl transferase polypeptide sequences identified using any of the alignments set forth in the group consisting of FIGS. 2a-2c, 3a-3c, 24a-24b, 25a-25b, 33a-33c, 34a-34b, and 35a-35b in a profile based model, excluding the amino acid sequences set forth in sequence listings in WO00/68393, WO 00/63391, WO 01/62781, or WO 02/33060 and do not comprise SEQ ID NOs 1-11, 43-45, 57-58, 61-62, and 91 from the present application.
Homogentisate prenyl transferase polypeptide sequences identified using any of the alignments set forth in FIGS. 2a-2c, 3a-3c, 24a-24b, 25a-25b, 33a-33c, 34a-34b, and 35a-35b in a profile based model, wherein said amino acid sequence is not derived from a nucleic acid molecule that is derived from Nostoc punctiforme, Anabaena, Synechocystis, Zea mays, Glycine max, Arabidopsis thaliana, Oryza sativa, wheat, leek, canola, cotton, or tomato.
Dependencies are inferred from the claim text.