Cyclin D polynucleotides polypeptides and uses thereof
AssigneePioneer Hi-Bred International, Inc.
InventorsKeith S. Lowe, Yumin Tao, William J. Gordon-Kamm, Carolyn A. Gregory, John A. McElver, George J. Hoerster, Sheila E. Maddock
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Abstract
The invention provides isolated polynucleotides and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants.
Claims58
A method for increasing transformation frequency in a plant cell comprising introducing into the plant cell a Cyclin D (CycD) polypeptide having transformation frequency-increasing activity.
A method for increasing transformation frequency comprising introducing a polynucleotide of interest into a plant cell containing a CycD polypeptide having transformation frequency-increasing activity.
A method for increasing transformation frequency of a plant cell comprising introducing into a plant cell an isolated CycD polynucleotide wherein said CycD polynucleotide is operably linked to a promoter capable of driving expression in the plant cell and wherein a CycD polypeptide is expressed from said CycD polynucleotide, said CycD polypeptide having transformation frequency-increasing activity.
A method for increasing transformation frequency of a plant cell comprising introducing into a plant cell an isolated CycD polynucleotide wherein said CycD polynucleotide is operably linked to a promoter capable of driving expression in said plant cell and wherein a CycD polypeptide is expressed from said CycD polynucleotide, said CycD polypeptide comprising cell cycle modulating activity.
The method of clam 16, further comprising growing the plant cell into a plant.
A method of increasing transformation frequency in a plant cell comprising introducing into the plant cell a CycD RNA or polypeptide, wherein said polypeptide comprises cell cycle modulating activity.
A method of modulating the cell cycle comprising transforming the plant cell with a recombinant expression cassette comprising an isolated CycD polynucleotide operably linked to a promoter, wherein said CycD polynucleotide encodes a CycD polypeptide which modulates cell cycle activity.
A method for providing a means of positive selection comprising expressing a CycD polypeptide in a plant cell and selecting for cells exhibiting a positive growth advantage, said polypeptide having transformation frequency increasing activity.
A method of modulating the level of CycD protein in a cell, comprising:
- (a) transforming a cell with a recombinant expression cassette comprising a CycD polynucleotide operably linked to a promoter;
- (b) growing the cell under cell-growing conditions for a time sufficient to induce expression of the polynucleotide sufficient to modulate CycD protein in the cell.
A method for transiently modulating the level of CycD protein in plant cells comprising introducing CycD polypeptides.
Dependencies are inferred from the claim text.