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US 2003/0213014 A1Published application
Root transcriptional factors and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorWesley B. Bruce
Life of this patent
Estimating the term…
Abstract
The invention provides isolated root transcriptional factor nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering root transcriptional factor levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.
Claims27
(Cancelled) An isolated nucleic acid comprising a member selected from the group consisting of:
- (a) a polynucleotide having at least 80% sequence identity, as determined by the GAP algorithm under default parameters, to a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NOS:
- 2, 6, and 10;
- (b) a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NOS:
- 2, 6, and 10;
- (c) a polynucleotide amplified from a Zea mays nucleic acid library using primers which selectively hybridize, under stringent hybridization conditions, to loci within a polynucleotide selected from the group consisting of SEQ ID NOS:
- 1, 5, and 9;
- (d) a polynucleotide which selectively hybridizes, under stringent hybridization conditions and a wash in 0.1×SSC at about 60° C. to about 65° C., to a polynucleotide selected from the group consisting of SEQ ID NOS:
- 1, 5, and 9;
- (e) a polynucleotide selected from the group consisting of SEQ ID NOS:
- 1, 5, and 9;
- (f) a polynucleotide which is complementary to a polynucleotide of (a), (b), (c), or (e);
- and
- (g) a polynucleotide comprising at least 25 contiguous nucleotides from a polynucleotide of (a), (b), (c), (d), (e), or (f).
10 more elements
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide that modulates root transcriptional activity, wherein the amino acid sequence of the polypeptide has at least 80% sequence identity across the full length of SEQ ID NO:
- 10, or
- (b) a nucleotide sequence that is complementary to the nucleotide sequence of (a)
- (c) a nucleotide sequence that has at least 80% sequence identity across the full length of SEQ ID NO:
- 9.
2 more elements
An isolated polynucleotide encoding a functional root transcriptional factor comprising a member selected from the consisting of:
- (a) a polynucleotide which selectively hybridizes under stringent hybridization conditions and a wash in 0.1×SSC at about 60° C. to about 65° C., to a polynucleotide comprising SEQ ID NO. 9;
- (b) a polynucleotide of SEQ ID NO:
- 9;
- (c) A polynucleotide which is complementary to a polynucleotide of (a) or (b).
1 more element
Dependencies are inferred from the claim text.