Fructose polymer synthesis in monocot plastids
OwnerPerry G. Caimi, Jonathan E. Lightnerinventors
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Abstract
This invention concerns methods for synthesis and accumulation of fructo-oligosaccharides in seeds of monocot plants by selective expression of a fructosyltransferase gene sequence. Selective expression includes coordination of timing, tissue specific expression and especially subcellular location. Successful transformants utilize sucrose to synthesize and accumulate fructo-oligosaccharides in the plastid of the cell, in established crops, without loss of co-products or concern for yield loss due to degradation during maturation, harvest or storage of the plant. Enhanced fructo-oligosaccharide production will benefit the fructose sweetener industry and add value to grain used for feed.
Claims17
A recombinant DNA construct comprising a tissue specific promoter, operably linked to a plastid targeting sequence, operably linked to a coding sequence for a fructosyltransferase gene such that said construct can transform a monocot cell to obtain the production of fructo-oligosaccharides in the plastid of said monocot cell.
A method of increasing fructo-oligosaccharide levels in a monocot, comprising inserting into a monocot cell a recombinant DNA construct comprising a tissue specific promoter, operably linked to a plastid targeting sequence, operably linked to a coding sequence for a fructosyltransferase gene, such that the construct can transform a monocot cell and the transformed monocot synthesizes and accumulates fructo-oligosaccharides.
A method of making a starch fructo-oligosaccharide blend comprising inserting into a monocot cell a recombinant DNA construct comprising a tissue specific promoter, operably linked to a plastid targeting sequence, operably linked to a coding sequence for a fructosyltransferase gene to produce a transgenic monocot plant, growing said plant, harvesting said plant, and extracting the fructo-oligosaccharide/starch blend from said harvested plant.
An isolated starch-fructo-oligosaccharide blend wherein the rheological properties of pasting temperature and final viscosity are independently altered relative to the pasting temperature and final viscosity of a starch solution substantially free of fructo-oligosaccharides, provided that said starch solution comprises substantially the same amount of starch as in said blend.
Dependencies are inferred from the claim text.