Transgenic high tryptophan plants
OwnerLisa M. Weaver, Tim N. Oulmassov +4inventors
InventorsLisa M. Weaver, Tim N. Oulmassov, Gabriela Vaduva, Jihong Liang, Marguerite J. Varagona, Tyamagondlu V. Venkatesh
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Abstract
The present invention provides a method for altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as human or animal food, seeds and progeny derived from these plants, are also provided.
Claims33
An isolated DNA molecule encoding an anthranilate synthase, wherein the DNA molecule encodes a protein substantially homologous to a protein selected from the group consisting of SEQ ID NOs:
- 66, 108, 109, 110, 111, 133, and 137.
A DNA construct comprising an expression cassette, wherein the expression cassette in operable linkage comprises (i) a heterologous promoter;
- (ii) a DNA molecule encoding a monomeric anthranilate synthase protein, wherein the monomeric anthranilate synthase comprises a single polypeptide comprising an anthranilate synthase α-domain and an anthranilate synthase β-domain;
- and (iii) a transcriptional terminator.
A DNA construct comprising a first expression cassette, wherein the first expression cassette in operable linkage comprises (i) a heterologous promoter;
- (ii) a DNA molecule encoding an anthranilate synthase α-domain protein;
- and (iii) a transcriptional terminator.
A method for altering the tryptophan content in a plant comprising:
- a. introducing into regenerable cells of a plant a transgene comprising an isolated DNA molecule encoding an anthranilate synthase wherein the isolated DNA is operably linked to a promoter functional in a plant cell, to yield transformed plant cells;
- and
- b. regenerating a plant from the transformed plant cells wherein the cells of the plant express the anthranilate synthase encoded by the isolated DNA in an amount effective to increase the tryptophan content in the plant relative to the tryptophan content in a second plant of the same or similar genetic background that does not include the transgene.
The method of claim 33, wherein the dicot is selected from the group consisting of soybean, cotton, and canola.
A method for making an animal feed or a human food comprising:
- a. introducing into regenerable cells of a plant a transgene comprising an isolated DNA molecule encoding an anthranilate synthase wherein the isolated DNA molecule is operably linked to a promoter functional in a plant cell, to yield transformed plant cells;
- and
- b. regenerating a plant from the transformed plant cells wherein the cells of the plant express the anthranilate synthase encoded by the isolated DNA molecule in an amount effective to increase the tryptophan content in the plant relative to the tryptophan content in a second plant of the same or similar genetic background that does not include the transgene.
Dependencies are inferred from the claim text.