Promoter and intron from maize actin depolymerizing factor
AssigneeDow AgroSciences LLC
InventorsBeth C. Rubin-Wilson, Kelley A. Smith
Life of this patent
Estimating the term…
Abstract
The promoter and an intron derived from maize actin depolymerizing factor, and genetic variants or deletions thereof are useful in controlling trangene expression in plants.
Claims18
An isolated DNA molecule comprising an ADF promoter.
An isolated DNA molecule comprising base pairs 1-734 of SEQ ID NO:1, or a fragment, genetic variant or deletion of such a sequence that retains the ability of functioning as a promoter in plant cells.
An isolated DNA molecule having a 20 base pair nucleotide portion identical in sequence to a 20 consecutive base pair portion of the sequence set forth in base pairs 1-734 of SEQ ID NO:1.
An isolated DNA molecule comprising ADF intron 1.
An isolated DNA molecule comprising base pairs 882-2161 of SEQ ID NO:1, or a fragment, genetic variant or deletion of such a sequence which retains the ability of enhancing expression when used with a promoter in plant cells.
An isolated DNA molecule having a 20 base pair nucleotide portion identical in sequence to a 20 consecutive base pair portion of the sequence set forth in base pairs 882-2161 of SEQ ID NO:1.
A DNA construct comprising a promoter operably linked to a heterologous nucleic acid sequence, wherein the promoter selectively hybridizes to SEQ ID NO:1.
A DNA construct comprising an intron linked to a heterologous nucleic acid sequence wherein said intron selectively hybridizes to SEQ ID NO:1.
A method of expressing a heterologous nucleic acid sequence in a plant comprising:
- a) introducing into a plant cell a vector comprising an ADF promoter operably linked to the heterologous nucleic acid sequence;
- and
- b) regenerating a plant from said cell.
A method of producing seed comprising:
- a) introducing into a plant cell a vector comprising an ADF promoter operably linked to a heterologous nucleic acid sequence;
- b) regenerating a plant from said cell;
- and
- c) sexually transmitting said ADF promoter operably linked to said heterologous nucleic acid sequence to progeny.
Dependencies are inferred from the claim text.