Mimetic peptides for an epitope of apolipoprotein B-100, concatemers of the peptides, and modified peptides, and compositions comprising the same
AssigneeHyo-Joon Kim
InventorsHyo-Joon Kim, Hae-Jung Joung
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Abstract
The present invention relates to a vaccine composition for treatment of obesity. More particularly, the present invention is directed to a vaccine composition which comprises a mimetic peptide epitope of apolipoprotein B-100, concatemers of said memetic peptide, and/or modified memetic peptides.
Claims24
A mimetic peptide for an epitope of apolipoprotein B-100, said peptide having the amino acid sequence of SEQ ID NO:1.
A mimetic peptide for an epitope of apolipoprotein B-100, said peptide having the amino acid sequence of SEQ ID NO:2.
A mimetic peptide for an epitope of apolipoprotein B-100, said peptide having the amino acid sequence of SEQ ID NO:3.
A composition for the treatment of obesity, said composition comprising a peptide selected from the group consisting of a peptide having the amino acid sequence of SEQ ID NO:1, a peptide having the amino acid sequence of SEQ ID NO:2, a peptide having the amino acid sequence of SEQ ID NO:3, and mixtures thereof admixed with a carrier.
A composition for the treatment of obesity, said composition comprising a concatemer of a peptide selected from the group consisting of a peptide having the amino acid sequence of SEQ ID NO:1, a peptide having the amino acid sequence of SEQ ID NO:2, a peptide having the amino acid sequence of SEQ ID NO:3, and mixtures thereof admixed with a carrier.
A method of treating obesity in a mammal comprising administering a composition by intradermal injection wherein in said composition comprises a mimetic peptide for an epitope of apolipoprotein B-100 selected from the group consisting of a peptide having the amino acid sequence of SEQ ID NO:1, a peptide having the amino acid sequence of SEQ ID NO:2, a peptide having the amino acid sequence of SEQ ID NO:3, and mixtures thereof admixed with a carrier.
A method of treating obesity in a mammal comprising administering a composition by intradermal injection wherein in said composition comprises a concatemer of a mimetic peptide for an epitope of apolipoprotein B-100 selected from the group consisting of a peptide having the amino acid sequence of SEQ ID NO:1, a peptide having the amino acid sequence of SEQ ID NO:2, a peptide having the amino acid sequence of SEQ ID NO:3, and mixtures thereof admixed with a carrier.
A process for preparing a mimetic peptide for an epitope of apolipoprotein B-100 comprising:
- i) inserting into a vector a nucleic acid encoding an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3;
- ii) transforming host cells with the vector obtained in i);
- iii) culturing said transformed host cells under conditions that permit expression of the inserted nucleic acid and formation of the encoded mimetic peptide;
- and
- iv) isolating said mimetic peptide from the host cell.
A process for preparing concatemer of a mimetic peptide for an epitope of apolipoprotein B-100 comprising:
- i) inserting into a vector a nucleic acid encoding a concatemer of an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3;
- ii) transforming host cells with the vector obtained in i);
- iii) culturing said transformed host cells under conditions that permit expression of the inserted nucleic acid and formation of the encoded mimetic peptide;
- and
- iv) isolating said mimetic peptide from the host cell.
A nucleic acid encoding a polypeptide comprising four (4) mimetic peptides for the epitope of apolipoprotein B-100 linked consecutively, wherein each mimetic peptide comprises the amino acid sequence of SEQ ID NO:1.
An expression vector comprising a nucleic acid encoding a polypeptide comprising four (4) mimetic peptides for the epitope of apolipoprotein B-100 linked consecutively, wherein each mimetic peptide comprises the amino acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.