Serum-free media for chondrocytes and methods of use thereof
AssigneeGenzyme Corporation
InventorLiesbeth Maria E. Brown
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Abstract
The present invention provides defined serum-free cell culture media useful in culturing fibroblasts, especially articular chondrocytes, that avoids problems inherent in the use of serum-containing media. The defined media comprise platelet-derived growth factor (PDGF), and chemically defined lipids, or combinations of these compounds. In another aspect, the present invention also provides tissue culture methods that comprise incubating chondrocytes in the defined serum free media. The methods enhance attachment and proliferative expansion of chondrocytes seeded at low density while maintaining their redifferentiation potential.
Claims49
A method for ex vivo propagation of chondrocytes, comprising the step of incubating the chondrocytes with a culture medium comprising a basal medium, PDGF, and one or more lipids selected from the group consisting of stearic acid, myristic acid, oleic acid, linoleic acid, palmitic acid, palmitoleic acid, arachidonic acid, linolenic acid, cholesterol, and alpha-tocopherol acetate.
A method for ex vivo propagation of chondrocytes, comprising the step of incubating the chondrocytes with a culture medium comprising cDRF, PDGF, stearic acid, myristic acid, oleic acid, linoleic acid, palmitic acid, palmitoleic acid, arachidonic acid, linolenic acid, cholesterol, and alpha-tocopherol acetate.
A serum-free medium comprising a basal medium, PDGF, stearic acid, myristic acid, oleic acid, linoleic acid, palmitic acid, palmitoleic acid, arachidonic acid, linolenic acid, cholesterol, and alpha-tocopherol acetate.
A composition comprising a chondrocyte and a culture medium comprising a basal medium, PDGF, and one or more lipids selected from the group consisting of stearic acid, myristic acid, oleic acid, linoleic acid, palmitic acid, palmitoleic acid, arachidonic acid, linolenic acid, cholesterol, and alpha-tocopherol acetate.
Dependencies are inferred from the claim text.