Minicell-based delivery agents
OwnerRoger A. Sabbadini, Robert Klepper +1inventors
InventorsRoger A. Sabbadini, Robert Klepper, Mark W. Surber
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Abstract
The invention provides compositions and methods for the production of achromosomal and anucleate cells useful for applications such as diagnositic and therapeutic uses, as well as research tools and agents for drug discovery.
Claims12
A minicell comprising a biologically active compound, wherein said minicell displays a ligand or binding moiety, wherein said ligand or binding moiety is part of a fusion protein comprising a first polypeptide that comprises at least one transmembrane domain or at least one membrane anchoring domain and a second polypeptide that comprises a binding moiety, and said minicell is a poroplast, spheroplast or protoplast.
A eubacterial minicell comprising a biologically active compound, wherein said minicell displays a binding moiety, wherein said binding moiety is selected from the group consisting of (a) a eukaryotic membrane protein;
- (b) an archeabacterial membrane protein;
- (c) an organellar membrane protein;
- and (d) a fusion protein, said fusion protein comprising a first polypeptide, said first polypeptide comprising at least one transmembrane domain or at least one membrane anchoring domain;
- and a second polypeptide, wherein said second polypeptide is not derived from a eubacterial protein and is neither a His tag nor a glutathione-S-transferase polypeptide, and wherein said polypeptide comprises a binding moiety.
Dependencies are inferred from the claim text.