Passivation of nerve agents by surface modified enzymes stabilized by non-covalent immobilization on robust, stable particles
OwnerAlok Singh, Mehran Pazirandeh +1inventors
InventorsAlok Singh, Mehran Pazirandeh, Paul E. Schoen
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Abstract
Enzymes are modified by incorporating anchor sites for linking the enzymes to a target surface without destroying the catalytic activity of the enzymes. A a stable carrier to accommodate and bind the selected enzyme is constructed, and the enzyme is non-covalently liked to the carrier, generally through metal salts of iminodiacetate
Claims14
A surface modified enzyme comprising an enzyme genetically engineered to include a stabilizing group at the N-terminus, which stabilizing group is used to immobilize the enzyme onto a polymerized vesicle or inorganic surface.
A method for stabilizing enzymes comprising:
- genetically engineering an enzyme to include a stabilizing amino acid substitution;
- copolymerizing an amphiphile containing a salt selected from the group consisting of metal salts of iminodiacetic acid, nitrilotriacetic acid, and mixtures thereof with other polymerizable amphiphiles to form vesicles;
- binding the genetically engineered enzyme to the salts on the outer surface of the vesicles.
A method for stabilizing enzymes comparing:
- genetically engineering an enzyme to include a stabilizing amino acid substitution;
- attaching said stabilized enzyme to salt groups selected from the group consisting of metal salts of iminodiacetic acid, metal salts of nitrilotriacetic acid, and mixtures thereof on the surface of a particular inorganic carrier.
Dependencies are inferred from the claim text.