Back to results
US 2003/0211573 A1Published application
Galactosylation of recombinant glycoproteins
OwnerThomas Ryllinventor
Life of this patent
Estimating the term…
Abstract
Enhanced glycosylation of glycoprotein expressed in host cells having enhanced CAD activity.
Claims9
A process for producing glycoprotein in a cell, the process comprising:
- expressing glycoprotein in cells having enhanced CAD activity;
- and thereby producing glycoproteins having enhanced glycosylation.
A process for producing glycoprotein in a cell, the process comprising the steps of:
- selecting cells that are resistant to a CAD inhibitor and expressing glycoprotein in said resistant cells, wherein said expressing produces glycoprotein having enhanced glycosylation.
A host cell for expression of glycosylated protein, the host cell comprising:
- a heterologous nucleic acid sequence encoding a glycosylated protein;
- and
- enhanced CAD activity as compared to host cells.
A galactosylated protein produced by a process comprising:
- expressing a heterologous nucleic acid sequence encoding the protein in a host cell having enhanced CAD activity as compared to control host cells.
A galactosylated protein produced by a process comprising:
- expressing a heterologous nucleic acid sequence encoding the protein in a host cell selected for resistance to CAD inhibitors.
Dependencies are inferred from the claim text.