Monooxygenase assays
OwnerSharat Singh, Ahmed Chenna +2inventors
InventorsSharat Singh, Ahmed Chenna, Hossein Salimi-Moosavi, Ian Gibbons
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Abstract
Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.
Claims4
A kit comprising a cytochrome P-450 enzyme, at least one P-450 enzyme substrate comprising an ether group bonded to a methylene, which is oxidized to an aldehyde by said P-450 enzyme, and a fluorescent hydrazine.
Dependencies are inferred from the claim text.