Multiplex cytokine analysis
OwnerTimothy Johann, Sheila Iraoinventors
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Abstract
The invention provides methods and compositions for the simultaneous, quantitative detection of cytokines in a sample. The methods use a solid phase array comprising a plurality of different antibodies arrayed in corresponding discrete array elements and specific for a corresponding plurality of different cytokines. The invention also provides methods of manufacturing and using the arrays to for the simultaneous, quantitative detection of cytokines in samples.
Claims20
An array comprising at least five different antibodies arrayed in corresponding discrete array elements and specific for corresponding five different cytokines.
An array comprising at least ten different antibodies arrayed in corresponding discrete array elements and specific for corresponding ten different cytokines,
- wherein the array is a microarray wherein the elements are arrayed at least 1000 elements per square centimeter,
- wherein the cytokines are GM-CSF, IL1α, IL1β, IL2, IL4, IL6, IL7, IL8, IL10 , IL12 and TNFα,
- wherein each antibody is specific for and bound to a different one of the corresponding cytokines, and
- wherein the antibodies are monoclonal.
Dependencies are inferred from the claim text.