Quality control reagents for nucleic acid microarrays
OwnerRobert C Getts, James M Kadushin +1inventors
InventorsRobert C Getts, James M Kadushin, Ari Zak
Life of this patent
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Abstract
Disclosed are reagents for conducting quality control reactions on microarrays of nucleic acids and kits containing the reagents, along with directions for conducting the reactions with the components in the kits. Also disclosed are methods of preparing the kits as well as using them to conduct the quality control reactions. A preferred reagent embodiment is illustrated in FIG. 3.
Claims25
A kit for conducting quality control reactions on a microarray of nucleic acids, comprising:
- a container comprising a first buffer solution comprising a first reagent comprising a nucleic acid matrix carrying a detectable label, said matrix having attached thereto an oligonucleotide that binds nucleic acid contained on any position on the microarray;
- and
- directions for conducting the quality control reactions with said first reagent and the nucleic acids on the microarray.
A kit for conducting quality control reactions on a microarray of nucleic acids, comprising:
- a first container comprising a first buffer solution comprising a nucleic acid matrix carrying a detectable label;
- and
- directions for producing a reagent by attaching to said matrix an oligonucleotide probe having a first end portion attachable to said matrix and a second end portion that binds nucleic acid on any position on the microarray, and conducting the quality control reactions with the reagent and the nucleic acids on the microarray.
A kit for conducting quality control reactions on a microarray of nucleic acids, comprising:
- a first container comprising a first buffer solution comprising a nucleic acid matrix carrying a detectable label;
- a second container comprising a second buffer solution comprising an oligonucleotide probe having a first end portion attachable to said matrix and a second end portion that binds nucleic acid on any position on the microarray;
- and
- directions for attaching said oligonucleotide probe to said matrix to prepare to first reagent and for conducting the quality control reactions with the first reagent and the nucleic acids on the microarray.
A method for preparing a kit for conducting quality control reactions on a microarray of nucleic acids, comprising:
- providing a container comprising a buffer solution comprising a reagent comprising a nucleic acid matrix carrying a detectable label, said matrix having attached thereto an oligonucleotide that binds nucleic acid contained on any position on the microarray;
- providing directions for conducting the quality control reactions with said reagent and the nucleic acids on the microarray;
- and
- packaging the container and the directions in the form of a kit.
A method for preparing a kit for conducting quality control reactions on a microarray of nucleic acids, comprising:
- providing a container comprising a buffer solution comprising a nucleic acid matrix carrying a detectable label;
- providing directions for preparing a reagent by attaching to said matrix an oligonucleotide having a first end portion attachable to said matrix and a second end portion that binds nucleic acid on the microarray, and conducting the quality control reactions with the reagent and the nucleic acids on any position on the microarray;
- and
- packaging the first container and the directions in the form of a kit.
A method for preparing a kit for conducting quality control reactions on a microarray of nucleic acids, comprising:
- providing a first container comprising a first buffer solution comprising a nucleic acid matrix carrying a detectable label;
- providing a second container comprising a second buffer solution comprising an oligonucleotide having a first end portion attachable to said matrix and a second end portion that binds nucleic acid on any position on the microarray;
- providing directions for attaching said oligonucleotide to said matrix to prepare a reagent and for conducting the quality control reactions with the reagent and the nucleic acids on the microarray;
- and
- packaging the first container, the second container and the directions in the form of a kit.
A method for conducting quality control reactions on a microarray of nucleic acids, comprising:
- providing the microarray of nucleic acids;
- providing a reagent comprising a nucleic acid matrix carrying a detectable label, said matrix having attached thereto an oligonucleotide that binds nucleic acid contained on any position on the microarray;
- contacting the reagent with the microarray;
- and
- detecting the label as an indication of presence or type of nucleic acid on the microarray.
A method for conducting quality control reactions on a microarray of nucleic acids, comprising:
- providing a nucleic acid matrix carrying a detectable label and an oligonucleotide probe having a first end portion attachable to said matrix and a second end portion that binds nucleic acid on the microarray;
- preparing a reagent by attaching the oligonucleotide probe to said matrix;
- contacting the reagent with the microarray;
- and
- detecting the label as an indication of presence or type of nucleic acid on the microarray.
Dependencies are inferred from the claim text.