Monomers and polymers having energy absorbing moieties of use in desorption/ionization of analytes
AssigneeCiphergen Biosystems, Inc.
InventorNaotaka Kitagawa
Life of this patent
Estimating the term…
Abstract
The present invention provides polymerizable monomers that incorporate moieties derived from energy absorbing molecules (EAM). The invention also provides polymers that are based on the monomers. The polymers have unique properties that make them ideally suited for use in diverse analyses, including desorption/ionization mass spectrometry of analytes. The invention also provides a device that incorporates the polymeric compositions of the inventions, methods of using the device to detect, quantify and identify analytes, and a method of preparing a device of the invention.
Claims60
A polymerizable monomer comprising:
- (a) a polymerizable moiety;
- and
- (b) a photo-reactive moiety comprising an aryl nucleus having a substituent thereon, said substituent comprising a carbonyl or carboxyl group conjugated to the 1-system of said aryl nucleus.
A polymeric material comprising a photo-reactive polymer that absorbs photo-irradiation from a high fluence source to generate thermal energy, and transfers said thermal energy to allow desorption and ionization of an analyte in operative contact with said photo-reactive polymer.
A device comprising:
- (a) a substrate having a surface;
- and
- (b) a polymeric material attached to said surface, wherein said polymeric material comprises a photo-reactive polymer that absorbs photo-irradiation from a high fluence source to generate thermal energy, and transfers said thermal energy to allow desorption and ionization of an analyte in operative contact with said photo-reactive polymer.
A method of making a device comprising:
- (a) contacting a surface of a substrate with a polymer precursor comprising a first photoreactive polymerizable monomer:
- (b) polymerizing said polymer precursor, thereby forming a layer of a photo-reactive polymer;
- and
- (c) immobilizing said layer of photo-reactive polymer on said surface.
A method of making a device comprising:
- (a) contacting a surface of a substrate with a polymeric material comprising a photo-reactive polymer that absorbs photo-irradiation from a high fluence source to generate thermal energy, and transfers said thermal energy to allow desorption and ionization of an analyte in operative contact with said photo-reactive polymer;
- and
- (b) immobilizing said polymeric material on said surface, thereby forming a layer of said polymeric material on said surface.
A method of detecting an analyte comprising:
- (a) providing a device comprising:
- (i) a substrate having a surface;
- and
- (ii) a polymeric material attached to said surface, wherein said polymeric material comprises a photo-reactive polymer that absorbs photo-irradiation from a high fluence source to generate thermal energy, and transfers said thermal energy to allow desorption and ionization of an analyte in operative contact with said photo-reactive polymer;
- (b) contacting an analyte with the polymeric material on the surface;
- and
- (c) interrogating the surface of device with photo-irradiation from a high fluence source and detecting the analyte by gas phase ion spectrometry.
A kit comprising:
- (a) a substrate comprising a surface;
- (b) a container comprising a polymerizable monomer comprising:
- (i) a polymerizable moiety;
- and
- (ii) a photo-reactive a moiety comprising an aryl nucleus having a substituent thereon, said substituent comprising a carbonyl or carboxyl group conjugated to the c-system of said aryl nucleus.
Dependencies are inferred from the claim text.