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US 2003/0207451 A1Published application
Methods, compositions, and kits for enhancing oligonucleotide-mediated nucleic acid sequence alteration using compositions comprising a histone deacetylase inhibitor, lambda phage beta protein, or hydroxyurea
AssigneeUniversity of Delaware
InventorsEric B. Kmiec, Hetal Parekh-Olmedo, Erin E. Brachman
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Abstract
Improved methods, compositions, and kits for oligonucleotide-mediated nucleic acid sequence alteration are presented.
Claims30
A method of oligonucleotide-mediated targeted sequence alteration of a nucleic acid, the method comprising:
- combining a target nucleic acid in the presence of cellular repair proteins with a sequence-altering targeting oligonucleotide;
- and
- either adding lambda beta protein additionally to said combination or first contacting the cells having said cellular repair proteins with an HDAC inhibitor or hydroxyurea.
A composition, comprising:
- an oligonucleotide, said oligonucleotide capable, when combined in the presence of cellular repair proteins with a target nucleic acid, of effecting targeted sequence alteration therein;
- and
- cellular repair proteins, said cellular repair proteins derived from a cell prior-contacted with an HDAC inhibitor or hydroxyurea.
A composition, comprising:
- an oligonucleotide, said oligonucleotide capable, when combined in the presence of cellular repair proteins with a target nucleic acid, of effecting targeted sequence alteration therein;
- and
- lambda beta protein.
A kit, comprising:
- an oligonucleotide;
- and
- cellular repair proteins, said cellular proteins derived from a cell prior-contacted with an HDAC inhibitor or hydroxyurea.
A kit, comprising:
- an oligonucleotide;
- and
- lambda beta protein.
Dependencies are inferred from the claim text.