Infectious papillomavirus pseudoviral particles
OwnerDouglas R. Lowy, John T. Schiller +1inventors
InventorsDouglas R. Lowy, John T. Schiller, Richard B. Roden
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Abstract
The invention provides an infectious papillomavirus pseudoviral particle useful in gene transfer comprising: (a) a papillomavirus vector DNA which comprises an E2 binding site and an expression cassette comprising a gene and a sequence controlling expression of said gene; and (b) a papillomavirus capsid which comprises L1 and L2 structural proteins, such that said capsid encapsidates said vector DNA, wherein said gene is derived from a first biological species and said L1 structural protein is derived from a second biological species and said first biological species is different from said second biological species.
Claims18
An infectious papillomavirus pseudoviral particle comprising:
- (a) a papillomavirus vector DNA which comprises an E2 binding site and an expression cassette comprising a gene and a sequence controlling expression of said gene;
- and
- (b) a papillomavirus capsid which comprises L1 and L2 structural proteins, such that said capsid encapsidates said vector DNA.
A method of making infectious papillomavirus pseudoviral particles comprising:
- (a) providing a cell line which expresses papillomavirus E2 DNA binding protein and L1 and L2 structural proteins;
- (b) transforming said cell line with a papillomavirus vector DNA which comprises an E2 binding site and an expression cassette comprising a gene and a sequence controlling expression of said gene, wherein said papillomavirus E2 binding site is a cognate binding site of said E2 DNA binding protein;
- (c) providing conditions for the encapsidation of said vector DNA by a capsid which comprises said L1 and L2 structural proteins to generate said particles;
- and
- (d) harvesting said particles.
An infectious papillomavirus pseudoviral particle for use as a medicament upon infecting cells of a human in vivo, wherein said particle comprises:
- (a) a papillomavirus vector DNA which comprises an E2 binding site and an expression cassette comprising a gene and a sequence controlling expression of said gene, wherein said gene encodes a therapeutic protein and said cells express a therapeutically effective amount thereof;
- and
- (b) a papillomavirus capsid which comprises L1 and L2 structural proteins, such that said capsid encapsidates said vector DNA.
An infectious papillomavirus pseudoviral particle for use as a vaccine upon infecting cells of a human in vivo, wherein said particle comprises:
- (a) a papillomavirus vector DNA which comprises an E2 binding site and an expression cassette comprising a gene and a sequence controlling expression of said gene, wherein said gene encodes an immunogenic protein and said cells express an immunogenically effective amount thereof;
- and
- (b) a papillomavirus capsid which comprises L1 and L2 structural proteins, such that said capsid encapsidates said vector DNA.
A second infectious papillomavirus pseudoviral particle, which differs from a first infectious papillomavirus pseudoviral particle, each particle for use as a vaccine upon infecting cells of a human in vivo, wherein each particle comprises:
- (a) a papillomavirus vector DNA which comprises an E2 binding site and an expression cassette comprising a gene and a sequence controlling expression of said gene, wherein said gene encodes an immunogenic protein and said cells express an immunogenically effective amount thereof;
- and
- (b) a papillomavirus capsid which comprises L1 and L2 structural proteins, such that said capsid encapsidate said vector DNA;
- such that said second infectious papillomavirus pseudoviral particle differs from said first infectious papillomavirus pseudoviral particle in that said second is a different serotype from said first.
Dependencies are inferred from the claim text.