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OwnerErhard Kopetzki, Karl-Peter Hopfner +2inventors
InventorsErhard Kopetzki, Karl-Peter Hopfner, Wolfram Bode, Robert Huber
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A process for the recombinant production of a protease is characterized by a) transforming a host cell with a recombinant nucleic acid which codes for a zymogenic precursor of a protease containing an autocatalytic cleavage site which does not occur naturally, wherein the active form of the said protease recognizes this cleavage site and cleaves the precursor to form the active protease, b) culturing the host cell in such a way that the zymogenic precursor of the protease is formed in the host cell in the form of inclusion bodies, c) isolating the inclusion bodies and renaturation under such conditions that the protease part of the zymogenic precursor is formed in its natural conformation and d) autocatalytic cleavage of the renatured zymogenic precursor to produce the active protease. This process is suitable for providing recombinant proteases in a simple manner and in large amounts.
A process for the recombinant production of a serine protease comprising:
An autocatalytically cleavable zymogenic precursor of a serine protease characterized by having a naturally occuring cleavage site replaced by an autocatalytic cleavage site which does not occur naturally.
A process for the recombinant production of an autocatalytically cleavable zymogenic precursor of a serine protease which contains no autocatalytic cleavage site in its naturally occurring form, said process comprising:
A process for the recombinant production of inclusion bodies which contain an autocatalytically cleavable zymogenic precursor of a serine protease, said protease characterized by containing no autocatalytic cleavage site in its naturally occurring form, said process comprising:
A recombinant, autocatalytically cleavable precursor of a serine protease which contains no autocatalytic cleavage site in its naturally occurring form, said precursor prepared by the process comprising:
Dependencies are inferred from the claim text.