US 2003/0207341 A1 · Methods for determining compounds capable of inhibiting the activities of the Ha-ras oncogene

Abstract

This invention provides a method for determining whether a compound is capable of suppressing ras functions comprising: (a) contacting an effective amount of the compound with Ha-ras transformed cloned rat embryo fibroblast cells under conditions permitting the compound to suppress ras functions in the cells; and (b) determining the expression or inhibition of certain indicator gene or genes, thereby determining whether the compound is capable of suppressing ras function. This invention further provides the determined compound and a pharmaceutical composition comprising the compound and a pharmaceutically acceptable carrier. This invention also provides methods for generating transcriptional switched Ha-ras transformed cloned rat embryo fibroblast cells. This invention also provides the generated cells and different uses of the cells.

Claims21

Dependent claims

A method for determining whether a compound is capable of suppressing the ras functions comprising:

(a) contacting an effective amount of the compound with Ha-ras transformed cloned rat embryo fibroblast cells under conditions permitting the compound to suppress the ras functions in the cells;
and
(b) determining the expression of lysyl oxidase, the expression of lysyl oxidase indicating that the compound is capable of suppressing the ras functions.

Dependencies are inferred from the claim text.

Methods for determining compounds capable of inhibiting the activities of the Ha-ras oncogene

Life of this patent

Methods for determining compounds capable of inhibiting the activities of the Ha-ras oncogene

Life of this patent

Abstract

Claims21