PCAM-1 marker protein, nucleic acid sequences encoding PCAM-1 and immunoassays for detection of PCAM-1
AssigneePhiladelphia Health and Education Corporation
InventorsMark Stearns, Youji Hu
Life of this patent
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Abstract
The present invention provides a cancer marker protein, PCAM-1, and polynucleotides which identify and encode this protein. Detection of this marker is useful in diagnosing and prognosticating cancer in a patient. Also provided are expression vectors and host cells for expression of PCAM-1 as well as antibodies raised against the PCAM-1 protein. In addition, a Monte Carlo-like screening assay for identification of specific 8 mer sequences which selectively bind proteins in a crude extrract of tissues, cells or other biological fluids is provided.
Claims16
A Monte Carlo like screening assay for identification of proteins in a selected tissue comprising producing random 8 mer double stranded DNA sequences and employing protein binding assays to identify the 8 mer sequence which binds a protein produced in the selected tissue.
An isolated, purified nucleic acid sequence which binds PCAM-1 protein.
An isolated, purified nucleic acid sequence which encodes a PCAM-1 polypeptide or hybridizes with a gene encoding a PCAM-1 polypeptide.
An isolated, purified PCAM-1 polypeptide.
An expression vector comprising a nucleic acid sequence which encodes a PCAM-1 polypeptide or hybridizes with a gene encoding a PCAM-1 polypeptide.
A method for diagnosing and prognosticating cancer in a patient comprising obtaining a biological sample from a patient and detecting or monitoring PCAM-1 protein levels in the biological sample wherein the presence of PCAM-1 protein in the biological sample is indicative of the presence of or progression of cancer in the patient.
A kit for diagnosing and prognosticating cancer in a patient comprising:
- (a) a PCAM-1 standard;
- and
- (b) a means for detecting PCAM-1 protein in biological samples.
Dependencies are inferred from the claim text.