Assay for the detection of factors that modulate the expression of INGAP
AssigneeThe Procter & Gamble Company
InventorsDavid A. Taylor-Fishwick, Aaron I. Vinik
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Abstract
A reporter construct contains mammalian INGAP 5′-regulatory region or a fragment thereof, a minimal promoter element from mammalian INGAP or a heterologous promoter, and a reporter gene. The reporter construct can be used to screen for agents which alone or in combination up-regulate or down-regulate reporter gene expression. Alternatively, the reporter construct can be used to screen for agents that bind to the hamster INGAP 5′-regulatory region or a fragment thereof.
Claims17
An isolated nucleic acid sequence of SEQ ID NO:
- 2.
An isolated nucleic acid sequence comprising at least about nucleotides 1-3137 of SEQ ID NO:
- 2 (SEQ ID NO:
- 23).
A reporter construct, comprising:
- a. a nucleotide sequence which encodes a detectable product;
- and
- b. a regulatory region nucleotide sequence linked to the 5′ end of nucleotide sequence that encodes a detectable product, wherein the regulatory region nucleotide sequence comprises of one or more regions chosen from nucleotides 1-3137 of SEQ ID NO:
- 2.
A method for modulating INGAP expression in a mammal in need thereof, comprising administering to the mammal an effective amount of a factor that stimulates INGAP expression in the said mammal.
Dependencies are inferred from the claim text.