US 2003/0207265 A1 · Method of making protein arrays

Abstract

Disclosed are methods of producing immobilized arrays of proteins. Included are methods for producing high density arrays of nucleic acids, amplifying arrays, and replicating such arrays. The nucleic acid molecules present on the support, whether amplified or not, are then expressed to produce proteins which are immobilized to the nucleic acid upon production or can be can be immobilized directly to the support. Alternatively, proteins can be bound to the nucleic acid molecules to produce protein arrays of the present invention. Arrays produced by the disclosed methods may include both nucleic acids and proteins or the nucleic acids can be removed from the array leaving the proteins. The disclosed methods also include replication of protein arrays in which a subset of the proteins that are produced can be transferred to an additional support where they are then immobilized.

Claims8

Dependent claims

A method of producing an array of proteins comprising,

a) providing a first nucleic acid array comprising nucleic acid molecules immobilized to a support,
b) expressing the nucleic acid molecules to produce proteins,
c) immobilizing the proteins to the support.

Dependencies are inferred from the claim text.

Facts

Application

09/767,764Filed 23 Jan 2001

Priority

10 Oct 1997

Published

6 Nov 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12Q 1/6837
Enzymatic or biochemical coupling of nucleic acids to a solid phase: using probe arrays or probe chips
G01N 33/547
Synthetic resin: with antigen or antibody attached to the carrier via a bridging agent
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms
G01N
Investigating or analysing materials by determining their chemical or physical properties

Family

Application60/061,511Us provisional application · filed 10 Oct 1997

Application60/076,570Us provisional application · filed 2 Mar 1998

Application09/143,014Continuation in part · filed 28 Aug 1998

Application09/267,496Continuation in part · filed 12 Mar 1999

Application09/522,732Continuation in part · filed 10 Mar 2000

Application09/767,764Continuation in part · filed 23 Jan 2001

US 6,432,360 B1Related patent

US 6,485,944 B1Related patent

US 6,511,803 B1Related patent

US 2003/0207265 A1This patent

From the US filing history. Foreign family members aren’t listed.

Method of making protein arrays

Life of this patent

Method of making protein arrays

Life of this patent

Abstract

Claims8