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US 2003/0207249 A1Published application
Connection of cells to substrates using association pairs
OwnerOren E. Beske, Simon Goldbardinventors
Life of this patent
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Abstract
The invention provides systems, including methods, apparatus, compositions, and kits, for connection of cells or cell structures to substrates, such as coded particles or microplates, utilizing association pairs that are chemically reactive or specifically bind one another.
Claims46
A method of analyzing cells, comprising:
- connecting a first member of an association pair to an external region of the cells;
- connecting a second member of the association pair to coded particles of at least two classes, each class having a different code;
- joining the first member to the second member to connect the cells to the coded particles;
- and
- performing an assay on at least one of the cells, or a portion thereof, connected to at least one of the coded particles.
2 more elements
A method of analyzing cells, comprising:
- selecting cells having a first member of an association pair connected to an external region of the cells;
- selecting coded particles having a second member of the association pair connected to a region of the coded particles accessible to cells, the coded particles being of at least two classes, each class having a different code;
- placing one or more coded particles from each of the at least two classes in a plurality of compartments;
- joining the first member to the second member to connect the cells to the coded particles;
- and
- performing an assay on at least one of the cells, or a portion thereof, connected to at least one of the coded particles.
3 more elements
A method of analyzing cells, comprising:
- fragmenting the cells to produce cell fragments;
- connecting a first member of an association pair to the cells or cell fragments;
- connecting a second member of the association pair to coded particles of at least two classes, each class having a different code;
- joining the first member to the second member to selectively connect a subset of the cell fragments to the coded particles;
- and
- performing an assay on at least one of the cell fragments connected to at least one of the coded particles.
3 more elements
A method of analyzing cells, comprising:
- connecting a first member of an association pair to a glycan disposed in an external region of the cells;
- connecting a second member of the association pair to coded particles of at least two classes, each class having a different code;
- joining the first member to the second member to connect the cells to the coded particles;
- and
- performing an assay on at least one of the cells, or a portion thereof, connected to at least one of the coded particles.
2 more elements
A method of preparing cells for multiplexed cellular assays, comprising:
- connecting a first member of a chemically reactive pair to cells in an external region of the cells;
- providing coded particles of at least two classes, each class having a different code, the coded particles being connected to a second member of the chemically reactive pair;
- and
- forming a covalent bond between the first member and the second member to connect the cells, or fragments thereof, to the coded particles.
1 more element
A kit for connecting cells to particles, comprising:
- a reagent configured to covalently connect a first member of an association pair to an external region of cells;
- and
- a set of coded particles of at least two classes, each class having a different code, the coded particles being connected to a second member of the association pair, the second member being configured to be joined to the first member.
Dependencies are inferred from the claim text.