Method and compositions for isolating metastatic cancer cells, and use in measuring metastatic potentatial of a cancer thereof
AssigneeThe Research Foundation of State University of New York
InventorWen-Tien Chen
Life of this patent
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Abstract
The present invention relates to novel methods and compositions for detection and isolation of cancer cells with metastatic potential. The invention further relates to assays for measuring the metastatic potential of such cancer cells and drug screening assays for the identification of agents having anti-metastatic potential. The present invention further provides methods and compositions for inhibiting the metastatic potential of cancer cells by modulating the activity of serine integral membrane proteases [(SIMP) consisting of seprase and dipedidyl peptidase IV (DPPIV)]expressed on the surface of metastasizing cancer cells.
Claims21
A method for detecting the presence of cancer cells in a subject comprising:
- (a) inoculating a sample derived from said subject onto a matrix wherein said matrix is coated with adhesion molecules;
- (b) incubating the sample for a time sufficient to allow adhesion of cancer cells to the cell-adhesion matrix;
- and
- (c) detecting the presence of cancer cells bound to the matrix,
A method for determining the metastatic potential of cancer cells derived from a cancer subject comprising:
- (a) inoculating the cancer cells derived from the cancer subject onto a matrix wherein said matrix is coated with adhesion molecules;
- (b) incubating the cancer cells for a time sufficient to allow adhesion, ingestion or invasion of the matrix by the cancer cells to occur;
- and
- (c) detecting adherence, ingestion or invasion of the matrix by the cancer cells
- wherein a detection of cancer cell adherence, ingestion or invasion of the matrix is an indicator of cancer cells with metastatic potential.
A method for identifying an agent that inhibits the metastatic potential of cancer cells comprising:
- (a) contacting the cancer cells and either a test agent or a vehicle control with a matrix coated with adhesion molecules;
- (b) incubating the matrix for a time sufficient for adhesion and ingestion of the matrix by the cancer cells to occur;
- and
- (c) detecting the adhesion and ingestion of the matrix by the cancer cells,
- wherein a decrease in the adhesion and ingestion of the matrix by the cancer cells in the presence of the test agent, as compared to the adhesion and ingestion of the matrix by the cancer cells in the presence of a vehicle control, identifies a compound that inhibits metastases formation.
A method for inhibiting the metastatic activity of cancer cells in a subject comprising the administration of a seprase inhibitor.
A method for inhibiting the metastatic activity of cancer cells in a subject comprising the administration of a dipeptidyl peptidase IV inhibitor.
A method for identifying a nucleic acid that inhibits the metastatic potential of cancer cells comprising:
- (a) introduction of the nucleic acid molecule into a cancer cell sample;
- (b) inoculating said cancer cell sample onto a matrix coated with an adhesion molecule;
- (c) incubating the matrix and cancer cell sample for a time sufficient for adhesion and ingestion of the matrix by the cancer cells to occur;
- and
- (d) detecting the adhesion and ingestion of the matrix by the cancer cells,
- wherein a decrease in the adhesion and ingestion of the matrix by the cancer cells containing the nucleic acid molecule, as compared to cancer cells not containing the nucleic acid molecule, identifies a nucleic acid molecule capable of inhibiting the metastatic potential of cancer cells.
A method for removal of cancer cells from a sample derived from a cancer subject comprising contacting said sample with a matrix coated with an adhesion molecule for a sufficient amount of time to permit the adherence of cancer cells to the cell-adhesion matrix.
A method for removal of cancer cells from the blood of a subject comprising contacting said blood with a matrix coated with an adhesion molecule for a sufficient amount of time to permit the adherence of cancer cells to the matrix.
A method for removal of cancer cells from the bone marrow of a subject comprising contacting said bone marrow with a cell-adhesion matrix coated with an adhesion molecule for a sufficient amount of time to permit the adherence of cancer cells to the cell-adhesion matrix.
Dependencies are inferred from the claim text.