Method for the detection of dyes in fluorescence microscopy
AssigneeCarl Zeiss Jena GmbH
InventorsRalf Wolleschensky, Gunter Moehler
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Abstract
A method for optical detection of characteristic quantities of the wavelength-dependent behavior of an illuminated specimen, such as the emission behavior and/or absorption behavior, preferably the fluorescence and/or enzyme fluorescence comprises determining at least one spectral centroid and/or a maximum of the emission radiation and/or of the absorbed radiation. An arrangement for performing the method is disclosed.
Claims86
A method for optical detection of characteristic quantities of the wavelength-dependent behavior of an illuminated specimen, such as the emission behavior and/or absorption behavior, preferably the fluorescence and/or luminescence and/or phosphorescence and/or enzyme-active light emission and/or enzyme-active fluorescence, comprising:
- determining at least one spectral centroid and/or a maximum of the emission radiation and/or of the absorbed radiation.
An arrangement for optical detection of characteristic quantities of the wavelength-dependent behavior of an illuminated specimen, particularly the emission behavior and/or absorption behavior, preferably the fluorescence and/or luminescence and/or phosphorescence and/or enzyme-active light emission and/or enzyme-active fluorescence, comprising:
- means for determining at least one spectral centroid and/or a maximum of the emission radiation and/or of the absorbed radiation.
Dependencies are inferred from the claim text.