Novel agrobacterium-mediated plant transformation method
OwnerCharles L. Armstrong, Jyoti R. Routinventors
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Abstract
The present invention relates to a novel transformation system for generating transformed plants with lower copy inserts and improved transformation efficiency. In particular, the invention relates to the use of Agrobacterium growth inhibiting agents during the Agrobacterium-mediated transformation process that suppress Agrobacterium growth and reduce T-DNA transfer to the target plant genome.
Claims66
A method of transforming a plant cell or plant tissue using an Agrobacterium mediated process comprising the steps of:
- inoculating a transformable plant cell or tissue with Agrobacterium containing at least one genetic component capable of being transferred to the plant cell or tissue in the presence of at least one growth inhibiting agent;
- co-culturing the transformable plant cell or tissue after inoculation in a media capable of supporting growth of plant cells or tissue expressing the genetic component, said media not containing a growth inhibiting agent;
- selecting transformed plant cells or tissue;
- and
- regenerating a transformed plant expressing the genetic component from the selected transformed plant cells or tissue.
A method of transforming a plant cell or plant tissue using an Agrobacterium mediated process comprising the steps of:
- inoculating a transformable plant cell or tissue with Agrobacterium containing at least one genetic component capable of being transferred to the plant cell or tissue;
- co-culturing the transformable plant cell or tissue after inoculation in a media capable of supporting growth of plant cells or tissue expressing the genetic component, said media further containing a growth inhibiting agent;
- selecting transformed plant cells or tissue;
- and
- regenerating a transformed plant expressing the genetic component from the selected transformed cells or tissue.
A method of transforming a plant cell or plant tissue using an Agrobacterium mediated process comprising the steps of:
- inoculating a transformable plant cell or tissue with Agrobacterium containing at least one genetic component capable of being transferred to the plant cell or tissue in the presence of at least one growth inhibiting agent;
- co-culturing the transformable plant cell or tissue after inoculation in a media capable of supporting growth of the plant cells or tissue expressing the genetic component, said media further containing a growth inhibiting agent;
- selecting transformed plant cells or tissue;
- and
- regenerating a transformed plant expressing the genetic component from the selected transformed cells or tissue.
Dependencies are inferred from the claim text.