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AssigneesPhiladelphia Health and Education Corporation, University of Medicine and Dentistry of New Jersey
InventorsIra B. Black, Dale L. Woodbury, Darwin J. Prockop, Emily Schwarz
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The present invention relates to methods of inducing differentiation of mammalian bone marrow stromal cells into neuronal cells by contacting marrow stromal cells with a neuronal differentiation-inducing compounds. Neuronal differentiation-inducing compounds of the invention include anti-oxidants such as, but not limited to, beta-mercaptoethanol, dimethylsulfoxide, butylated hydroxyanisole, butylated hydroxytoluene, ascorbic acid, dimethylfumarate, and n-acetylcysteine. Once induced to differentiate into neuronal cells, the cells can be used for cell therapy, gene therapy, or both, for treatment of diseases, disorders, or conditions of the central nervous system.
A method of inducing differentiation of an isolated marrow stromal cell into a neuronal cell, said method comprising contacting said isolated marrow stromal cell with at least one neuronal differentiation-inducing compound, thereby inducing differentiation of said isolated marrow stromal cell into a neuronal cell.
A method of producing an isolated neuronal cell, said method comprising isolating a marrow stromal cell, contacting said marrow stromal cell with a neuronal differentiation-inducing compound wherein said compound induces said isolated marrow stromal cell to differentiate into an isolated neuronal cell, thereby producing said isolated neuronal cell.
A method of treating a human patient having a disease, disorder or condition of the central nervous system, said method comprising obtaining a bone marrow sample from a human donor, isolating stromal cells from said bone marrow sample, inducing said stromal cells to differentiate into isolated neuronal cells, and administering said isolated neuronal cells to the central nervous system of said human patient, wherein the presence of said isolated neuronal cells in said central nervous system of said human patient effects treatment of said disease, disorder or condition.
A method of treating a human patient in need of neuronal cells, said method comprising obtaining marrow stromal cells from a human patient, propagating said marrow stromal cells in culture under conditions that induce their differentiation into neuronal cells, transplanting said neuronal cells into said human patient in need of said neuronal cells, thereby treating said human patient in need of neuronal cells.
An isolated neuronal cell made by a method of inducing differentiation of an isolated marrow stromal cell, said method comprising contacting said isolated marrow stromal cell with at least one neuronal differentiation-inducing compound, thereby inducing differentiation of said isolated marrow stromal cell into said neuronal cell.
An isolated neuronal cell made by a method of inducing differentiation of an isolated marrow stromal cell, said method comprising contacting said isolated marrow stromal cell with at least one neuronal differentiation-inducing compound, thereby inducing differentiation of said isolated marrow stromal cell in said neuronal cell, wherein said neuronal cell is further transfected with an isolated nucleic acid encoding a therapeutic protein, and further wherein when said protein is expressed in said cell said protein serves to effect treatment of a disease, disorder, or condition of the central nervous system.
An isolated neuronal cell made by a method of producing an isolated neuronal cell, said method comprising isolating a marrow stromal cell, contacting said marrow stromal cell with a neuronal differentiation inducing compound, wherein said compound induces said isolated marrow stromal cell to differentiate into said isolated neuronal cell, thereby producing said isolated neuronal cell.
An isolated neuronal cell made by a method of producing an isolated neuronal cell, said method comprising isolating a marrow stromal cell, contacting said marrow stromal cell with a neuronal differentiation inducing compound, wherein said compound induces said isolated marrow stromal cell to differentiate into said isolated neuronal cell, thereby producing said isolated neuronal cell, wherein said neuronal cell is further transfected with an isolated nucleic acid encoding a therapeutic protein, and further wherein when said protein is expressed in said cell, said protein serves to effect treatment of a disease, disorder, or condition of the central nervous system.
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