US 2003/0203408 A1Published application

Computer-implemented method for identifying peaks in electropherogram data

OwnerStephen Williams, Paul E. Theobald +1inventors

InventorsStephen Williams, Paul E. Theobald, Mengxiana Tang

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Abstract

The invention provides methods and algorithms for measuring one or more analytes in a sample by using a plurality of releasable molecular tags attached to binding compounds specific for the analytes of interest. After binding compounds specifically bind to their respective analytes to form complexes, the molecular tags of the binding compounds forming such complexes are cleaved and released, while the molecular tags of those binding compounds not forming such complexes are not released. The released molecular tags are then electrophorectically separated along with one or more electrophorectic standards to generate electropherogram data, and the identity of each molecular tag is determined by the location of its corresponding peak in such data relative to the one or more of the electrophorectic standards. In this way, distortions in the electropherogram data due to factors such as instrumentation differences, assay conditions, reagent variability, or the like, can be taken into account and experimental results from different electrophoresis systems, different assays, or the like may be compared.

Claims5

Dependent claims

A computer-readable product embodying a program for execution by a computer to identify a plurality of molecular tags by determining locations of peaks in electropherogram data correlated with such tags, the program comprising instructions for:

(a) reading electropherogram data from a storage medium, the electropherogram data obtained by electrophoretic separation of the plurality of molecular tags and one or more electrophoretic standards, each electrophoretic standard and molecular tag having a different electrophoretic mobility such that upon electrophoretic separation each electrophoretic standard and molecular tag forms a distinct peak in the electropherogram data, and each peak of such one or more electrophoretic standards and molecular tags having a migration interval and each migration interval having a mean;
(b) determining a peak location of at least one electrophorctic standard within a migration interval in the electropherogram data;
(c) determining peak locations of peaks within a migration interval in the electropherogram data closest to an electrophoretic standard or a qualified peak in a qualified peak set, the peak locations being relative to the location of the closest electrophoretic standard or qualified peak, and correlating with a molecular tag or an electrophoretic standard a peak within the migration interval whose location is closest to the mean of the migration interval;
(d) determining a peak signal-to-noise ratio of the peak and adding the peak to the qualified peak set if the peak signal-to-noise ratio is greater than or equal to 1.5;
and
(e) repeating steps (c) and (d) until a peak location is correlated to every molecular tag having a peak in the electropherogram data.

3 more elements

Dependencies are inferred from the claim text.