Proportional amplification of nucleic acids
OwnerYanxiang Cao, David J. Lockhart +2inventors
InventorsYanxiang Cao, David J. Lockhart, Rui Mei, Xing Su
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Abstract
The proportional amplification of nucleic acids can increase the amount of nucleic acids while preserving the relative abundance of the individual nucleic acid species, or portions thereof, in the original sample. A proportionally amplified nucleic acid preparation may be analyzed in a gene expression monitoring system, preferably involving a nucleic acid probe array.
Claims75
A method for the proportional amplification of nucleic acid, said method comprising:
- creating fragments of a single-stranded DNA population;
- synthesizing double-stranded DNA from said fragments of a single-stranded DNA population;
- and
- producing multiple copies of sense RNA from said double-stranded DNA.
A method for the proportional amplification of nucleic acid, said method comprising:
- creating fragments of a double-stranded DNA population;
- and
- synthesizing multiple copies of said fragments of a double-stranded DNA population.
A method for the proportional amplification of nucleic acid, said method comprising:
- synthesizing multiple copies of a double-stranded DNA population;
- and
- creating fragments of said multiple copies of a double-stranded DNA population.
Dependencies are inferred from the claim text.